{"id":3409,"date":"2024-03-26T07:02:21","date_gmt":"2024-03-25T23:02:21","guid":{"rendered":"http:\/\/oncolab.cn\/index.php\/2024\/03\/26\/nature-cancer%ef%bc%9a%e8%82%bf%e7%98%a4%e7%9b%b8%e5%85%b3%e9%ab%93%e7%b3%bb%e7%bb%86%e8%83%9e%e4%b8%8a%e7%9a%84sirp%ce%b1%e5%9c%a8%e7%bb%93%e7%9b%b4%e8%82%a0%e7%99%8c%e4%b8%ad%e7%8b%ac%e7%ab%8b\/"},"modified":"2024-03-26T07:02:21","modified_gmt":"2024-03-25T23:02:21","slug":"nature-cancer%ef%bc%9a%e8%82%bf%e7%98%a4%e7%9b%b8%e5%85%b3%e9%ab%93%e7%b3%bb%e7%bb%86%e8%83%9e%e4%b8%8a%e7%9a%84sirp%ce%b1%e5%9c%a8%e7%bb%93%e7%9b%b4%e8%82%a0%e7%99%8c%e4%b8%ad%e7%8b%ac%e7%ab%8b","status":"publish","type":"post","link":"http:\/\/oncolab.cn\/index.php\/2024\/03\/26\/nature-cancer%ef%bc%9a%e8%82%bf%e7%98%a4%e7%9b%b8%e5%85%b3%e9%ab%93%e7%b3%bb%e7%bb%86%e8%83%9e%e4%b8%8a%e7%9a%84sirp%ce%b1%e5%9c%a8%e7%bb%93%e7%9b%b4%e8%82%a0%e7%99%8c%e4%b8%ad%e7%8b%ac%e7%ab%8b\/","title":{"rendered":"Nature Cancer\uff1a\u80bf\u7624\u76f8\u5173\u9ad3\u7cfb\u7ec6\u80de\u4e0a\u7684Sirp\u03b1\u5728\u7ed3\u76f4\u80a0\u764c\u4e2d\u72ec\u7acb\u4e8e\u5176\u4e0eCD47\u76f8\u4e92\u4f5c\u7528\u6291\u5236\u6297\u80bf\u7624\u514d\u75ab"},"content":{"rendered":"
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\u6742\u5fd7\u540d\u79f0<\/strong>\uff1aNat Cancer.<\/span><\/p>\n

\u53d1\u8868\u65e5\u671f<\/strong>\uff1a2024.01.10<\/p>\n

DOI<\/strong>\uff1a10.1038\/s43018-023-00691-z.<\/p>\n

PubMed<\/strong>\uff1ahttps:\/\/pubmed.ncbi.nlm.nih.gov\/38200243\/<\/p>\n

\u5f71\u54cd\u56e0\u5b50<\/strong>\uff1a22.7<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u5185\u5bb9\u7b80\u4ecb<\/strong><\/p>\n<\/section>\n

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\u80bf\u7624\u4e2d\u7684\u514d\u75ab\u6291\u5236\u6027\u9ad3\u7cfb\u7ec6\u80de\u963b\u788d\u4e86\u514d\u75ab\u6cbb\u7597\u7684\u6548\u679c\uff0c\u4f46\u5177\u4f53\u673a\u5236\u5c1a\u672a\u660e\u786e\u3002\u901a\u8fc7\u5bf9\u7ed3\u76f4\u80a0\u764c\u7ec4\u7ec7\u8fdb\u884c\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\uff0c\u6211\u4eec\u53d1\u73b0\u4e0e\u6b63\u5e38\u7ec4\u7ec7\u76f8\u6bd4\uff0c\u80bf\u7624\u76f8\u5173\u7684\u5de8\u566c\u7ec6\u80de\u548c\u7c92\u7ec6\u80de\u6027\u9ad3\u7cfb\u6765\u6e90\u7684\u6291\u5236\u7ec6\u80de\u6570\u91cf\u589e\u52a0\u6700\u591a\uff0c\u5e76\u4e14\u5728\u6240\u6709\u514d\u75ab\u7ec6\u80de\u4e2d\u5c55\u793a\u4e86\u6700\u9ad8\u7684\u514d\u75ab\u6291\u5236\u7279\u5f81\u3002\u8fd9\u4e9b\u7ec6\u80de\u663e\u8457\u589e\u52a0\u4e86\u5305\u62ecSIRPA\u5728\u5185\u7684\u542b\u514d\u75ab\u53d7\u4f53\u916a\u6c28\u9178\u6291\u5236\u57fa\u5e8f\u53d7\u4f53\u7684\u8868\u8fbe\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0cSirpa-\/-<\/sup>\u5c0f\u9f20\u6bd4\u91ce\u751f\u578b\u5c0f\u9f20\u5bf9\u80bf\u7624\u8fdb\u5c55\u66f4\u5177\u62b5\u6297\u529b\u3002\u6b64\u5916\uff0cSirp\u03b1\u7f3a\u5931\u901a\u8fc7\u6269\u589e\u663e\u793a\u5f3a\u70c8\u6297\u80bf\u7624\u6d3b\u6027\u7684TAM_Ccl8hi<\/sup>\u548cgMDSC_H2-Q10hi<\/sup>\u4e9a\u7fa4\u91cd\u7f16\u7a0b\u4e86\u80bf\u7624\u5fae\u73af\u5883\u3002Sirpa-\/-<\/sup>\u5de8\u566c\u7ec6\u80de\u5c55\u73b0\u51fa\u5f3a\u70c8\u7684\u541e\u566c\u4f5c\u7528\u548c\u6297\u539f\u5448\u9012\u80fd\u529b\uff0c\u4ee5\u589e\u5f3aT\u7ec6\u80de\u7684\u6fc0\u6d3b\u548c\u589e\u6b96\u3002\u6b64\u5916\uff0cSirpa-\/-<\/sup>\u5de8\u566c\u7ec6\u80de\u901a\u8fc7Syk\/Btk\u4f9d\u8d56\u7684Ccl8\u5206\u6ccc\u4fc3\u8fdb\u4e86T\u7ec6\u80de\u7684\u62db\u52df\u3002\u56e0\u6b64\uff0cSirp\u03b1\u7f3a\u5931\u589e\u5f3a\u4e86\u5148\u5929\u548c\u9002\u5e94\u6027\u514d\u75ab\u6fc0\u6d3b\uff0c\u72ec\u7acb\u4e8eCD47\u7684\u8868\u8fbe\uff0c\u800cSirp\u03b1\u963b\u65ad\u53ef\u80fd\u662f\u63d0\u9ad8\u764c\u75c7\u514d\u75ab\u6cbb\u7597\u6548\u679c\u7684\u6709\u5e0c\u671b\u7684\u7b56\u7565\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u521b\u65b0\u6027\u53ca\u7814\u7a76\u610f\u4e49<\/strong><\/p>\n<\/section>\n

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\u8fd9\u7bc7\u6587\u7ae0\u7684\u6838\u5fc3\u521b\u65b0\u6027\u5728\u4e8e\u63ed\u793a\u4e86\u80bf\u7624\u76f8\u5173\u9ad3\u7cfb\u7ec6\u80de\u4e0a\u7684Sirp\u03b1\u5728\u7ed3\u76f4\u80a0\u764c\u4e2d\u6291\u5236\u6297\u80bf\u7624\u514d\u75ab\u7684\u65b0\u673a\u5236\uff0c\u8fd9\u4e00\u8fc7\u7a0b\u72ec\u7acb\u4e8e\u5176\u4e0eCD47\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u7814\u7a76\u901a\u8fc7\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6280\u672f\uff0c\u53d1\u73b0\u4e0e\u6b63\u5e38\u7ec4\u7ec7\u76f8\u6bd4\uff0c\u7ed3\u76f4\u80a0\u764c\u7ec4\u7ec7\u4e2d\u7684\u80bf\u7624\u76f8\u5173\u5de8\u566c\u7ec6\u80de\u548c\u7c92\u7ec6\u80de\u6027\u9ad3\u7cfb\u6765\u6e90\u6291\u5236\u7ec6\u80de\u663e\u8457\u589e\u591a\uff0c\u8868\u73b0\u51fa\u5f3a\u70c8\u7684\u514d\u75ab\u6291\u5236\u7279\u5f81\uff0c\u5e76\u5177\u6709\u9ad8\u8868\u8fbe\u542b\u514d\u75ab\u53d7\u4f53\u916a\u6c28\u9178\u6291\u5236\u57fa\u5e8f\u7684\u53d7\u4f53\uff0c\u5305\u62ecSIRPA\u3002<\/p>\n

\u8be5\u7814\u7a76\u8fdb\u4e00\u6b65\u8bc1\u660e\u4e86Sirpa\u57fa\u56e0\u6572\u9664\uff08Sirpa-\/-<\/sup>\uff09\u5c0f\u9f20\u76f8\u6bd4\u4e8e\u91ce\u751f\u578b\u5c0f\u9f20\u5bf9\u80bf\u7624\u8fdb\u5c55\u66f4\u5177\u62b5\u6297\u529b\u3002\u91cd\u8981\u7684\u662f\uff0cSirp\u03b1\u7f3a\u5931\u4e0d\u4ec5\u91cd\u65b0\u7f16\u7a0b\u4e86\u80bf\u7624\u5fae\u73af\u5883\uff0c\u901a\u8fc7\u6269\u589e\u5177\u6709\u5f3a\u70c8\u6297\u80bf\u7624\u6d3b\u6027\u7684\u7ec6\u80de\u4e9a\u7fa4\uff0c\u8fd8\u589e\u5f3a\u4e86\u5de8\u566c\u7ec6\u80de\u7684\u541e\u566c\u4f5c\u7528\u548c\u6297\u539f\u5448\u9012\u80fd\u529b\uff0c\u8fdb\u800c\u589e\u5f3aT\u7ec6\u80de\u7684\u6fc0\u6d3b\u548c\u589e\u6b96\u3002\u6b64\u5916\uff0cSirp\u03b1\u7f3a\u5931\u5de8\u566c\u7ec6\u80de\u8fd8\u80fd\u901a\u8fc7\u7279\u5b9a\u7684\u5206\u6ccc\u9014\u5f84\u4fc3\u8fdbT\u7ec6\u80de\u7684\u62db\u52df\uff0c\u4ece\u800c\u589e\u5f3a\u4e86\u5148\u5929\u548c\u9002\u5e94\u6027\u514d\u75ab\u53cd\u5e94\u3002<\/p>\n

\u7814\u7a76\u610f\u4e49\u5728\u4e8e\uff0c\u8fd9\u4e9b\u53d1\u73b0\u4e3a\u7ed3\u76f4\u80a0\u764c\u7684\u514d\u75ab\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u9776\u70b9\uff0c\u5373\u901a\u8fc7\u963b\u65adSirp\u03b1\u6765\u589e\u5f3a\u6297\u80bf\u7624\u514d\u75ab\u53cd\u5e94\uff0c\u4e3a\u764c\u75c7\u6cbb\u7597\u63d0\u4f9b\u4e86\u6f5c\u5728\u7684\u65b0\u7b56\u7565\u3002\u8fd9\u4e00\u53d1\u73b0\u4e0d\u4ec5\u6709\u52a9\u4e8e\u6df1\u5316\u6211\u4eec\u5bf9\u80bf\u7624\u514d\u75ab\u73af\u5883\u7684\u7406\u89e3\uff0c\u4e5f\u4e3a\u5f00\u53d1\u65b0\u7684\u514d\u75ab\u6cbb\u7597\u65b9\u6cd5\u63d0\u4f9b\u4e86\u79d1\u5b66\u4f9d\u636e\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6570\u636e\u56fe<\/strong><\/p>\n<\/section>\n

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\u56fe 1 | \u80bf\u7624\u4e2d\u5bcc\u96c6\u7684\u9ad3\u7cfb\u7ec6\u80de\u5c55\u73b0\u51fa\u6700\u9ad8\u7684\u514d\u75ab\u6291\u5236\u7279\u5f81\u3002<\/strong><\/p>\n

a\uff0c\u6765\u81ea\u4e09\u4f4d\u7ed3\u76f4\u80a0\u764c\uff08CRC\uff09\u60a3\u8005\u768462,288\u4e2a\u514d\u75ab\u7ec6\u80de\u7684t-SNE\u6295\u5f71\uff0c\u6839\u636e\u7ec6\u80de\u7c7b\u578b\uff08\u5de6\u4fa7\uff09\u548c\u7ec4\u7ec7\u7c7b\u578b\uff08\u53f3\u4fa7\uff09\u8fdb\u884c\u7740\u8272\u3002<\/p>\n

b\uff0c\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u4e2d\u5404\u4e2a\u6837\u672c\u548c\u7ec4\u7ec7\u7684\u767e\u5206\u6bd4\u3002CRC1\u3001CRC2\u548cCRC3\u4ee3\u8868\u4e09\u4f4d\u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u3002<\/p>\n

c\uff0c\u80bf\u7624\u7ec4\u7ec7\u4e0e\u6b63\u5e38\/\u764c\u65c1\u7ec4\u7ec7\u95f4\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u706b\u5c71\u56fe\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002\u91c7\u7528Bonferroni\u65b9\u6cd5\u8fdb\u884c\u591a\u91cd\u6bd4\u8f83\u6821\u6b63\u3002<\/p>\n

d\uff0c\u80bf\u7624\u6d78\u6da6\u514d\u75ab\u7ec6\u80de\uff08TIICs\uff09\u4e2d\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u514d\u75ab\u6291\u5236\u8bc4\u5206\u3002<\/p>\n

e\uff0cf\uff0c\u5de8\u566c\u7ec6\u80de\uff08e\uff09\u548c\u4e2d\u6027\u7c92\u7ec6\u80de\uff08f\uff09\u5728CRCN\u3001CRCP\u548cCRCT\u7ec4\u7ec7\u4e2d\u663e\u8457\u5bcc\u96c6\u7684\u7279\u5f81\u3002\u989c\u8272\u6df1\u5ea6\u4ee3\u8868-log10(q\u503c)\u3002TH\u7ec6\u80de\uff0c\u8f85\u52a9\u6027T\u7ec6\u80de\u3002<\/p>\n

g\uff0c\u6765\u81ea\u4e09\u4f4d\u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u768410,245\u4e2a\u9ad3\u7cfb\u7ec6\u80de\u7684t-SNE\u53ef\u89c6\u5316\uff0c\u6839\u636e\u63a8\u65ad\u7684\u7ec6\u80de\u4e9a\u7fa4\u8fdb\u884c\u7740\u8272\u3002<\/p>\n

h\uff0c\u6bcf\u4e2a\u9ad3\u7cfb\u7ec6\u80de\u4e9a\u7fa4\u4e2d\u5404\u4e2a\u6837\u672c\u548c\u7ec4\u7ec7\u7684\u6bd4\u4f8b\u3002<\/p>\n

i\uff0cj\uff0c\u4e0d\u540c\u7ec4\u7ec7\u4e2d\u5de8\u566c\u7ec6\u80de\uff08i\uff09\u548c\u4e2d\u6027\u7c92\u7ec6\u80de\uff08j\uff09\u4e2d\u514d\u75ab\u6291\u5236\u57fa\u56e0\u7684\u8868\u8fbe\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002\u7bb1\u7ebf\u56fe\u4e2d\u6807\u8bb0\u4e86\u6700\u5c0f\u503c\u3001\u6700\u5927\u503c\u3001\u4e2d\u5fc3\u3001\u8fb9\u754c\u4ee5\u53ca\u767e\u5206\u4f4d\u6570\u3002<\/p>\n

NS\uff0c\u4e0d\u663e\u8457\u3002CRCT\uff0c\u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u7684\u80bf\u7624\u7ec4\u7ec7\uff1bCRCN\uff0c\u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u7684\u6b63\u5e38\u7ec4\u7ec7\uff1bCRCP\uff0c\u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u7684\u764c\u65c1\u7ec4\u7ec7\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 2 | Sirp\u03b1\u57fa\u56e0\u7684\u7f3a\u5931\u6291\u5236\u5b9e\u4f53\u80bf\u7624\u7684\u53d1\u5c55\u3002<\/strong><\/p>\n

a\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 12\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 11\u53ea\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

b\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 6\u53ea\uff09\u548cClec4a2\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

c\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 6\u53ea\uff09\u548cSiglece\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 13\u53ea\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

d\uff0c\u643a\u5e26\u76ae\u4e0bMC38\u80bf\u7624\u7684\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 14\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 9\u53ea\uff09\u7684\u751f\u5b58\u5206\u6790\u3002<\/p>\n

e\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 6\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u4f53\u5185\u76ae\u4e0bLewis\u80ba\u764c\uff08LLC\uff09\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

f\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 9\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u4f53\u5185\u76ae\u4e0bHepa1-6\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

g\uff0ch\uff0c\u5728\u6d88\u9664\u521d\u6b21\u63a5\u79cd\u7684Hepa1-6\u80bf\u762445\u5929\u540e\uff0c\u518d\u6b21\u63a5\u79cd\u4e09\u500dHepa1-6\u7ec6\u80de\u7684\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 6\u53ea\uff09\u6216\u65e0\u80bf\u7624\u7684Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 17\u53ea\uff09\u7684\u80bf\u7624\u751f\u957f\uff08g\uff09\u548c\u751f\u5b58\u5206\u6790\uff08h\uff09\u3002<\/p>\n

i\uff0c\u5728\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 12\u4e2a\u6837\u672c\uff09\u6216Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 11\u4e2a\u6837\u672c\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u4e2d\uff0c\u5de8\u566c\u7ec6\u80de\u548cgMDSCs\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

j\uff0ck\uff0c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u8ba1\u7b97\u5f97\u51fa\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 12\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 11\u4e2a\u6837\u672c\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u4e2d\u7684\u5de8\u566c\u7ec6\u80de\u3001gMDSCs\u548cmMDSCs\u4e2d\u7684MHC-II\u8868\u8fbe\uff08j\uff09\u6216CD86\u8868\u8fbe\uff08k\uff09\u3002<\/p>\n

l\uff0c\u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u8ba1\u7b97\u5f97\u51fa\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 6\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u4e2a\u6837\u672c\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u4e2dCD4+ <\/sup>T\u7ec6\u80de\u548cCD8+ <\/sup>T\u7ec6\u80de\u7684\u767e\u5206\u6bd4\u3002\u6240\u6709\u6837\u672c\u5747\u6765\u81ea\u72ec\u7acb\u7684\u5c0f\u9f20\u3002\u7ed3\u679c(a,d,i\u2013l)\u6c47\u603b\u81ea\u4e24\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u7ed3\u679c(b,c,g,h)\u4ee3\u8868\u4e24\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e4b\u4e00\u3002\u7ed3\u679c(e,f)\u4ee3\u8868\u4e94\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e4b\u4e00\u3002\u8bef\u5dee\u6761\u8868\u793a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u91c7\u7528\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\uff08a\u2013c,e\u2013g,i\u2013l\uff09\u548c\u5bf9\u6570\u79e9\u68c0\u9a8c\uff08d,h\uff09\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
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<\/section>\n<\/section>\n
\n

\u56fe 3 | Sirp\u03b1\u7f3a\u5931\u963b\u6b62\u81ea\u53d1\u6027\u7ed3\u80a0\u764c\u7684\u53d1\u5c55\u3002<\/strong><\/p>\n

a\uff0cAOM\/DSS\u8bf1\u5bfc\u7684\u81ea\u53d1\u6027\u7ed3\u76f4\u80a0\u764c\uff08CRC\uff09\u6a21\u578b\u4e2d\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08WT\uff0cn = 21\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 15\u53ea\uff09\u7684\u751f\u5b58\u5206\u6790\u3002<\/p>\n

b\uff0cAOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d\u5927\u80a0\u80bf\u7624\u7684\u4ee3\u8868\u6027\u56fe\u7247\u3002<\/p>\n

c-f\uff0c\u5728AOM\/DSS\u8bf1\u5bfc\u7684CRC\u6a21\u578b\u4e2d\uff0c\u6d4b\u91cf\u91ce\u751f\u578b\u5c0f\u9f20\uff08n = 10\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 15\u53ea\uff09\u7684\u80bf\u7624\u8d1f\u8377\uff08c\uff09\uff0c\u80bf\u7624\u6570\u91cf\uff08d\uff09\uff0c\u603b\u80bf\u7624\u4f53\u79ef\uff08e\uff09\u548c\u5355\u4e2a\u80bf\u7624\u4f53\u79ef\uff08f\uff09\u3002<\/p>\n

g\uff0c\u7528\u82cf\u6728\u7cbe-\u4f0a\u7ea2\uff08H&E\uff09\u67d3\u8272\u5bf9\u91ce\u751f\u578b\u5c0f\u9f20\uff08\u6709\u80bf\u7624\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08\u65e0\u80bf\u7624\uff09\u7684\u7ed3\u80a0\u7ec4\u7ec7\u8fdb\u884c\u7684\u7ec4\u7ec7\u5b66\u5206\u6790\u7684\u4ee3\u8868\u6027\u56fe\u7247\u3002\u6bd4\u4f8b\u5c3a\uff0c50\u5fae\u7c73\u3002<\/p>\n

h\uff0c\u5728AOM\/DSS\u8bf1\u5bfc\u7684CRC\u6a21\u578b\u4e2d\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\uff08n = 10\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 10\u53ea\uff09\u7684\u7ed3\u80a0\u7ec4\u7ec7\u4e2d\u708e\u75c7\u7ec6\u80de\u6d78\u6da6\u7684\u8bc4\u5206\u3002<\/p>\n

i\uff0c\u5728WT\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4f53\u5185\u63a5\u79cdMC38-vector\u6216MC38-Cd47\u2212\/\u2212<\/sup>\u7ec6\u80de\u540e\uff0c\u76ae\u4e0b\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002WT + MC38-vector\uff08n = 6\u53ea\uff09\uff0cSirpa\u2212\/\u2212<\/sup> + MC38-vector\uff08n = 7\u53ea\uff09\uff0cWT + MC38-Cd47\u2212\/\u2212<\/sup>\uff08n = 6\u53ea\uff09\uff0cSirpa\u2212\/\u2212<\/sup> + MC38-Cd47\u2212\/\u2212<\/sup>\uff08n = 7\u53ea\uff09\u3002<\/p>\n

j\uff0c\u4f7f\u7528CD47\u6297\u4f53\u6cbb\u7597\uff08anti-CD47\uff0cn = 5\u53ea\uff09\u548c\u4e0d\u6cbb\u7597\uff08\u5bf9\u7167\u7ec4\uff1bn = 4\u53ea\uff09\u7684AOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d\u7684\u80bf\u7624\u8d1f\u8377\u3001\u80bf\u7624\u6570\u91cf\u3001\u80bf\u7624\u4f53\u79ef\u548c\u5355\u4e2a\u80bf\u7624\u4f53\u79ef\u3002<\/p>\n

\u7ed3\u679c(a,b,g,h)\u4ee3\u8868\u4e09\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e4b\u4e00\u3002\u7ed3\u679c(c-f)\u6c47\u603b\u81ea\u4e24\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u7ed3\u679c(i)\u4ee3\u8868\u56db\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e4b\u4e00\uff0c\u7ed3\u679c(j)\u6765\u81ea\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u6570\u636e\u8868\u793a\u4e3a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u4f7f\u7528\u5bf9\u6570\u79e9\u68c0\u9a8c(a)\u548c\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09(c-f,h-j)\u8fdb\u884c\u7edf\u8ba1\u5206\u6790\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
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<\/section>\n<\/section>\n
\n

\u56fe 4 | Sirp\u03b1\u7f3a\u5931\u91cd\u65b0\u7f16\u7a0b\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883\u4ee5\u4fc3\u8fdb\u6297\u80bf\u7624\u514d\u75ab\u3002<\/strong><\/p>\n

a\uff0c\u91c7\u7528t-SNE\u53ef\u89c6\u5316\u65b9\u6cd5\u5c55\u793a\u4e86\u6765\u81ea\u4e24\u53ea\u91ce\u751f\u578b\u5c0f\u9f20\u548c\u4e24\u53eaSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u7684AOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d11,516\u4e2a\u80bf\u7624\u6d78\u6da6\u514d\u75ab\u7ec6\u80de\uff08TIICs\uff09\uff0c\u6309\u7ec6\u80de\u7c7b\u578b\u8fdb\u884c\u7740\u8272\u3002<\/p>\n

b\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2d\u5404\u7ec6\u80de\u7c7b\u578b\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

c\uff0c\u4f7f\u7528QuSAGE\u6bd4\u8f83Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e0e\u91ce\u751f\u578b\u5c0f\u9f20\u4e2d\u5de8\u566c\u7ec6\u80de\uff08TAMs\uff0c\u4e0a\u56fe\uff09\u548c\u8089\u82bd\u80bf\u6027\u9ad3\u7ec6\u80de\uff08gMDSCs\uff0c\u4e0b\u56fe\uff09\u7684\u6297\u539f\u5448\u9012\u8bc4\u5206\u3001\u541e\u566c\u8bc4\u5206\u548c\u708e\u75c7\u53cd\u5e94\u8bc4\u5206\u3002x\u8f74\u4ee3\u8868\u57fa\u56e0\u96c6\u8bc4\u5206\u7684log2(\u500d\u6570\u53d8\u5316)\u3002<\/p>\n

d\uff0c\u91c7\u7528t-SNE\u53ef\u89c6\u5316\u65b9\u6cd5\u5c55\u793a\u4e86\u6765\u81ea\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u76847,842\u4e2a\u80bf\u7624\u6d78\u6da6\u7684\u9ad3\u7cfb\u7ec6\u80de\uff0c\u6309\u7ec6\u80de\u4e9a\u7fa4\u8fdb\u884c\u7740\u8272\u3002<\/p>\n

e\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2d\u6bcf\u4e2a\u9ad3\u7cfb\u7ec6\u80de\u4e9a\u7fa4\u7684\u7ec6\u80de\u6bd4\u4f8b\u3002<\/p>\n

f\uff0c\u901a\u8fc7scVelo\u548cCellRank\u63a8\u65ad\u7684\u5de8\u566c\u7ec6\u80de\u8c31\u7cfb\u7684\u53d1\u5c55\u8f68\u8ff9\u3002\u6bcf\u4e2a\u7c07\u7684\u997c\u56fe\u663e\u793a\u7ec6\u80de\u7fa4\u4f53\u5206\u5316\u4e3a\u7279\u5b9a\u7ec8\u7aef\u8c31\u7cfb\u7684\u6982\u7387\uff0c\u7bad\u5934\u6307\u793a\u8f68\u8ff9\u65b9\u5411\u3002<\/p>\n

g\uff0c\u5bf9\u5e94\u4e8e\u63a8\u65ad\u8c31\u7cfb\u7684\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2d\u5de8\u566c\u7ec6\u80de\u4e9a\u7fa4\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

h\uff0c\u4f7f\u7528QuSAGE\u6bd4\u8f83TAM3\u548cTAM2\u7684\u6297\u539f\u5448\u9012\u8bc4\u5206\u3001\u541e\u566c\u8bc4\u5206\u3001\u708e\u75c7\u53cd\u5e94\u8bc4\u5206\u548c\u8d8b\u5316\u56e0\u5b50\u6d3b\u6027\u8bc4\u5206\u3002<\/p>\n

i\uff0c\u901a\u8fc7scVelo\u548cCellRank\u63a8\u65ad\u7684\u8089\u82bd\u80bf\u6027\u9ad3\u7ec6\u80de\uff08gMDSC\uff09\u8c31\u7cfb\u7684\u53d1\u5c55\u8f68\u8ff9\u3002\u8bc6\u522b\u51fa\u4e24\u4e2a\u8c31\u7cfb\u3002\u56fe\u4f8b\u6ce8\u91ca\u4e0ef\u76f8\u540c\u3002<\/p>\n

j\uff0c\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2dgMDSC\u4e9a\u7fa4\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

k\uff0c\u4f7f\u7528QuSAGE\u6bd4\u8f83gMDSC6\/gMDSC7\u4e0egMDSC5\u7684\u6297\u539f\u5448\u9012\u8bc4\u5206\u3001\u541e\u566c\u8bc4\u5206\u3001\u708e\u75c7\u53cd\u5e94\u8bc4\u5206\u548c\u8d8b\u5316\u56e0\u5b50\u6d3b\u6027\u8bc4\u5206\u3002P\u503c\u662f\u4f7f\u7528QuSAGE\u4ece\u6982\u7387\u5bc6\u5ea6\u51fd\u6570\u4e2d\u63d0\u53d6\u7684\uff08c,h\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n
\n

\u56fe 5 | Sirp\u03b1\u7f3a\u5931\u589e\u5f3a\u4e86\u5de8\u566c\u7ec6\u80de\u529f\u80fd\u548cT\u7ec6\u80de\u6fc0\u6d3b\u3002<\/strong><\/p>\n

a,b\uff0c\u591a\u7cd6(I:C)\u6fc0\u6d3b\u7684\u91ce\u751f\u578b\uff08WT\uff0cn = 8\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\uff08n = 8\u4e2a\u6837\u672c\uff09\u9aa8\u9ad3\u6765\u6e90\u7684\u5de8\u566c\u7ec6\u80de\uff08BMDMs\uff09\uff08a\uff09\u6216\u80bf\u7624\u76f8\u5173\u5de8\u566c\u7ec6\u80de\uff08TAMs\uff09WT\uff08n = 3\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\uff08n = 4\u4e2a\u6837\u672c\uff09\uff08b\uff09\u5bf9MC38\u7ec6\u80de\u7684\u541e\u566c\u4f5c\u7528\u3002<\/p>\n

c\uff0c\u5728WT\uff08n = 17\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\uff08n = 14\u53ea\uff09\u3001WT + \u6297CSF1R\uff08n = 13\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup> + \u6297CSF1R\u7ec4\uff08n = 13\u53ea\uff09\u5c0f\u9f20\u4f53\u5185\uff0c\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

d\uff0c\u5728WT\uff08n = 5\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\uff08n = 8\u53ea\uff09\u3001WT + WT BMDMs\uff08n = 6\u53ea\uff09\u548cWT + Sirpa\u2212\/\u2212<\/sup> BMDM\u7ec4\uff08n = 6\u53ea\uff09\u5c0f\u9f20\u4f53\u5185\uff0c\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

e,f\uff0c\u5728Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u76f8\u6bd4\u4e8eWT\u5c0f\u9f20\u4e2d\uff0c\u80bf\u7624\u6d78\u6da6\u7684CD8+ T\u7ec6\u80de\uff08e\uff09\u548cCD4+ T\u7ec6\u80de\uff08f\uff09\u4e2d\u663e\u8457\u5bcc\u96c6\u7684\u7279\u5f81\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

g\uff0c\u5728WT\uff08n = 6\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\uff08n = 5\u53ea\uff09\u3001WT + \u6297CD8\uff08n = 5\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup> + \u6297CD8\u7ec4\uff08n = 6\u53ea\uff09\u5c0f\u9f20\u4f53\u5185\uff0c\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

h\uff0c\u5728WT\uff08n = 11\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\uff08n = 5\u53ea\uff09\u3001WT + \u6297CD4\uff08n = 6\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup> + \u6297CD4\u7ec4\uff08n = 5\u53ea\uff09\u5c0f\u9f20\u4f53\u5185\uff0c\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

i\uff0c\u901a\u8fc7CellChat\u8ba1\u7b97\u7684\u4efb\u610f\u4e24\u79cd\u7ec6\u80de\u7c7b\u578b\u95f4\u901a\u8fc7\u7ec6\u80de-\u7ec6\u80de\u63a5\u89e6\u4fe1\u53f7\u4f20\u9012\u7684\u524d10%\u663e\u8457\u914d\u4f53-\u53d7\u4f53\u5bf9\uff08L-R\u5bf9\uff09\u3002<\/p>\n

j\uff0c\u5728Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2d\uff0c\u6765\u81eamMDSCs\u3001TAMs\u3001cDCs\u548cgMDSCs\u5230CD4+ T\u7ec6\u80de\uff08\u5de6\u4fa7\uff09\u6216CD8+ T\u7ec6\u80de\uff08\u53f3\u4fa7\uff09\u7684\u7ec6\u80de-\u7ec6\u80de\u63a5\u89e6\u4fe1\u53f7\u4f20\u9012\u7684\u4e0a\u8c03L-R\u5bf9\u3002<\/p>\n

k\uff0c\u7531WT BMDMs\uff08n = 3\u4e2a\u6837\u672c\uff09\u6216Sirpa\u2212\/\u2212<\/sup> BMDMs\uff08n = 3\u4e2a\u6837\u672c\uff09\u9884\u5904\u7406OVA\u80bd\u540e\u5f15\u53d1\u7684OT-I T\u7ec6\u80de\u589e\u6b96\uff0c\u4ee5\u4e0d\u540c\u6bd4\u4f8b\u8fdb\u884c\u3002<\/p>\n

l\uff0c\u7531WT TAMs\uff08n = 5\u4e2a\u6837\u672c\uff09\u6216Sirpa\u2212\/\u2212<\/sup> TAMs\uff08n = 5\u4e2a\u6837\u672c\uff09\u9884\u5904\u7406OVA\u80bd\u540e\u5f15\u53d1\u7684OT-I T\u7ec6\u80de\u589e\u6b96\uff0c\u4ee510:1\u7684\u6bd4\u4f8b\u8fdb\u884c\u3002<\/p>\n

\u6240\u6709\u6837\u672c\u5747\u6765\u81ea\u72ec\u7acb\u5c0f\u9f20\u3002\u6570\u636e\u8868\u793a\u4e3a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\uff0c\u6c47\u603b\u81ea\u516b\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08a\uff09\u548c\u4e24\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08b,c\uff09\uff0c\u6216\u4ee3\u8868\u4e09\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e4b\u4e00\uff08d,g\uff09\uff0c\u4e24\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e4b\u4e00\uff08h,k\uff09\uff0c\u4ee5\u53ca\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08l\uff09\u3002\u4f7f\u7528\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u8ba1\u7b97P\u503c\uff08a\u2013d,g,h,k,l\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n
\n

\u56fe 6 | Sirp\u03b1\u7f3a\u5931\u7684\u5de8\u566c\u7ec6\u80de\u901a\u8fc7\u4f9d\u8d56Syk\/Btk\u7684Ccl8\u5206\u6ccc\u589e\u5f3aT\u7ec6\u80de\u62db\u52df\u3002<\/strong><\/p>\n

a\uff0c\u7ec6\u80de\u7c7b\u578b\u5bf9\u4e4b\u95f4\u5206\u6ccc\u4fe1\u53f7\u4f20\u9012\u7684\u524d10%\u663e\u8457\u914d\u4f53-\u53d7\u4f53\u5bf9\uff08L\u2013R\u5bf9\uff09\u3002<\/p>\n

b\uff0c\u4f7f\u7528QuSAGE\u6bd4\u8f83\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2dmMDSC\u3001TAM\u548cgMDSC\u7684\u8d8b\u5316\u56e0\u5b50\u6d3b\u6027\u8bc4\u5206\u3002<\/p>\n

c,d\uff0c\u5728\u9aa8\u9ad3\u6765\u6e90\u5de8\u566c\u7ec6\u80de\uff08BMDMs\uff09WT\uff08n = 10\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\uff08n = 10\u4e2a\u6837\u672c\uff09\uff08c\uff09\u6216TAMs WT\uff08n = 9\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\uff08n = 9\u4e2a\u6837\u672c\uff09\uff08d\uff09\u4f5c\u7528\u4e0b\uff0cCD8+ T\u7ec6\u80de\u6216CD4+ T\u7ec6\u80de\u7684\u500d\u589e\u53d8\u5316\u3002<\/p>\n

e\uff0c\u5728Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2d\uff0c\u6765\u81eamMDSC\u3001TAM\u548cgMDSC\u5230CD4\/CD8 T\u7ec6\u80de\u7684\u4e0a\u8c03\u5206\u6ccc\u4fe1\u53f7\u4f20\u9012\u7684L\u2013R\u5bf9\u3002<\/p>\n

f\uff0cCcl3\uff08n = 3\u4e2a\u6837\u672c\uff09\u3001Ccl4\uff08n = 4\u4e2a\u6837\u672c\uff09\u3001Ccl5\uff08n = 6\u4e2a\u6837\u672c\uff09\u3001Ccl8\uff08n = 9\u4e2a\u6837\u672c\uff09\u6216Ccl12\uff08n = 6\u4e2a\u6837\u672c\uff09\u62db\u52df\u7684\u813e\u810fCD4+<\/sup>\u6216CD8+<\/sup> T\u7ec6\u80de\u7684\u500d\u589e\u53d8\u5316\u3002<\/p>\n

g\uff0cCcl3\u3001Ccl4\u6216Ccl8\uff08n = 3\u4e2a\u6837\u672c\uff09\u62db\u52df\u7684CD4+\u6216CD8+\u80bf\u7624\u6d78\u6da6\u6dcb\u5df4\u7ec6\u80de\uff08TILs\uff09\u7684\u500d\u589e\u53d8\u5316\u3002<\/p>\n

h\uff0c\u5728WT\uff08n = 9\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u548c\u7ed9\u4e88Ccl3\uff08n = 5\u53ea\uff09\u3001Ccl4\uff08n = 6\u53ea\uff09\u6216Ccl8\uff08n = 5\u53ea\uff09\u7684WT\u5c0f\u9f20\u4f53\u5185\uff0c\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

i\uff0c\u4f7f\u7528\u53cd\u8f6c\u5f55qPCR\u6d4b\u8bd5Sirpa\u2212\/\u2212<\/sup> BMDMs\u5728\u65e0\u6291\u5236\u5242\uff08\u5bf9\u7167\uff09\u6216\u52a0\u5165Stat1\uff08n = 5\u4e2a\u6837\u672c\uff09\u3001Btk\uff08n = 7\u4e2a\u6837\u672c\uff09\u6216Syk\uff08n = 6\u4e2a\u6837\u672c\uff09\u6291\u5236\u5242\u60c5\u51b5\u4e0b\u7684Ccl8\u8868\u8fbe\u3002<\/p>\n

j\uff0cWT\u5c0f\u9f20\u4e2dSyk\u8868\u8fbe\u6c34\u5e73\u7684t-SNE\u53ef\u89c6\u5316\u3002<\/p>\n

k\uff0c\u5728AOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d\uff0cWT\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u7684mMDSCs\u3001TAMs\u548cgMDSCs\u4e2dSyk\u7684\u8868\u8fbe\u6c34\u5e73\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

l\uff0c\u5728\u63a5\u53d7\u65b0\u8f85\u52a9\u6297PD-1\u6cbb\u7597\u7684\u9ed1\u8272\u7d20\u7624\u60a3\u8005\u4e2d\uff0cSIRPA\/CD68\u7684\u6bd4\u7387\u3002\u975e\u590d\u53d1\uff08n = 8\u540d\u60a3\u8005\uff09\u548c\u590d\u53d1\uff08n = 5\u540d\u60a3\u8005\uff09\u3002rec\uff0c\u590d\u53d1\u3002<\/p>\n

m\uff0c\u5728AOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d\uff0cWT\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u7684mMDSCs\u3001TAMs\u548cgMDSCs\u4e2dPD-L1\uff08Cd274\uff09\u7684\u8868\u8fbe\u6c34\u5e73\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

n\uff0c\u5728WT\uff08n = 15\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\uff08n = 8\u53ea\uff09\u3001WT + \u6297PD-L1\uff08n = 12\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup> + \u6297PD-L1\u7ec4\uff08n = 11\u53ea\uff09\u5c0f\u9f20\u4f53\u5185\uff0c\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

\u6240\u6709\u6837\u672c\u5747\u6765\u81ea\u72ec\u7acb\u5c0f\u9f20\u3002\u6570\u636e\uff08\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\uff09\u6765\u81ea\u56db\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08c\uff09\u3001\u516d\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08f\uff09\u3001\u4e03\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08i\uff09\u3001\u4e24\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08d\u3001n\uff09\u30011\u4e2a\u72ec\u7acb\u5b9e\u9a8c(h)\u548c1\u4e2a\u72ec\u7acb\u5b9e\u9a8c(g)\u3002P\u503c\u91c7\u7528\u53cc\u5c3eStudent ‘s t\u68c0\u9a8c(c,d,f – i,n)\u8ba1\u7b97\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6269\u5c55\u6570\u636e \u56fe 1 | \u7ed3\u76f4\u80a0\u764c\u60a3\u8005\u80bf\u7624\u7ec4\u7ec7\u4e2d\u7684\u9ad3\u7cfb\u7ec6\u80de\u5c55\u73b0\u51fa\u4fc3\u8fdb\u80bf\u7624\u7684\u7279\u5f81\u3002<\/strong><\/p>\n

a. \u901a\u8fc7\u8868\u8fbe\u6c34\u5e73\u7740\u8272\u7684\u7ec6\u80de\u7c7b\u578b\u7279\u5f02\u6027\u57fa\u56e0\u7684t-SNE\u53ef\u89c6\u5316\u3002<\/p>\n

b. \u901a\u8fc7scDC\u8ba1\u7b97\u5f97\u51faCRCN\u3001CRCP\u548cCRCT\u7ec4\u7ec7\u4e2d\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u6bd4\u4f8b\u3002\u901a\u8fc7scDC\u4e2d\u7684GLM\u6a21\u578b\u8fdb\u884c\u81ea\u52a9\u91cd\u91c7\u6837\u6765\u8ba1\u7b97p\u503c\u3002<\/p>\n

c. \u6bcf\u4e2a\u9ad3\u7cfb\u7ec6\u80de\u7c07\u4e2d\u524d3\u4e2a\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u70ed\u56fe\u3002\u989c\u8272\u4ee3\u8868\u6bcf\u4e2a\u7c07\u4e2d\u57fa\u56e0\u5e73\u5747\u8868\u8fbe\u7684\u6807\u51c6\u5316\u503c\u3002<\/p>\n

d, e. \u5b8f\u89c2\u89c2\u6d4b\u5de8\u566c\u7ec6\u80de\uff08d\uff09\u548c\u4e2d\u6027\u7c92\u7ec6\u80de\uff08e\uff09\u5728\u80bf\u7624\u7ec4\u7ec7\u4e0e\u6b63\u5e38\/\u764c\u65c1\u7ec4\u7ec7\u4e4b\u95f4\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u706b\u5c71\u56fe\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002\u7ea2\u70b9\u548c\u84dd\u70b9\u5206\u522b\u4ee3\u8868\u5728\u80bf\u7624\u7ec4\u7ec7\u548c\u6b63\u5e38\/\u764c\u65c1\u7ec4\u7ec7\u4e2d\u9ad8\u8868\u8fbe\u7684\u57fa\u56e0\u3002\u4f7f\u7528Bonferroni\u65b9\u6cd5\u5bf9\u591a\u91cd\u6bd4\u8f83\u8fdb\u884c\u6821\u6b63\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n
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\u6269\u5c55\u6570\u636e\u56fe 2 | \u5728\u5927\u6570\u636e\u4e2d\uff0c\u80bf\u7624\u6d78\u6da6\u7684\u9ad3\u7cfb\u7ec6\u80de\u5c55\u793a\u51fa\u6700\u9ad8\u7684\u514d\u75ab\u6291\u5236\u7279\u5f81\u3002<\/strong><\/p>\n

a\u2013c. \u4ecesyn26844071\u3001GSE188711\u548cGSE178341\u4e2d\uff0c131\u4f4d\u7ed3\u76f4\u80a0\u764c\uff08CRC\uff09\u60a3\u8005\u7684455,959\u4e2a\u514d\u75ab\u7ec6\u80de\u7684UMAP\u6295\u5f71\uff0c\u6309\u7ec6\u80de\u7c7b\u578b\uff08a\uff09\u3001\u7ec4\u7ec7\uff08b\uff09\u548cTNM\u5206\u671f\uff08c\uff09\u7740\u8272\u3002<\/p>\n

d. \u80bf\u7624\u7ec4\u7ec7\u4e2d\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u514d\u75ab\u6291\u5236\u8bc4\u5206\u3002<\/p>\n

e. \u80bf\u7624\u76f8\u5173\u5de8\u566c\u7ec6\u80de\uff08TAM\uff09\u7279\u5f02\u57fa\u56e0\u663e\u8457\u5bcc\u96c6\u7684\u7279\u5f81\u3002\u989c\u8272\u6df1\u5ea6\u4ee3\u8868-Log10 (p\u503c)\u3002<\/p>\n

f-k. \u6765\u81ea\u6240\u6709131\u4f4dCRC\u60a3\u8005\u7684\u5de8\u566c\u7ec6\u80de\uff08f\uff09\u548c\u4e2d\u6027\u7c92\u7ec6\u80de\uff08g\uff09\uff0c\u4ee5\u53caI\u671f\uff08h\uff09\u3001II\u671f\uff08i\uff09\u3001III\u671f\uff08j\uff09\u548cIV\u671f\uff08k\uff09\u5de8\u566c\u7ec6\u80de\u5728\u80bf\u7624\u548c\u6b63\u5e38\u7ec4\u7ec7\u4e2d\u7684\u514d\u75ab\u6291\u5236\u57fa\u56e0\u8868\u8fbe\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n
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\u6269\u5c55\u6570\u636e\u56fe 3 | Sirp\u03b1\u57fa\u56e0\u6572\u9664\u5c0f\u9f20\u7684\u57fa\u672c\u4fe1\u606f\u53ca\u5176\u57fa\u7ebf\u514d\u75ab\u7279\u5f81\u3002<\/strong><\/p>\n

a. Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u7684\u751f\u6210\u65b9\u6848\u3002<\/p>\n

b, c. \u901a\u8fc7\u514d\u75ab\u5370\u8ff9\uff08Western blot\uff09\uff08b\uff09\u548c\u6d41\u5f0f\u7ec6\u80de\u672f\uff08FACS\uff09\uff08c\uff09\u68c0\u6d4b\u91ce\u751f\u578b\uff08WT\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u9aa8\u9ad3\u6765\u6e90\u5de8\u566c\u7ec6\u80de\uff08BMDMs\uff09\u4e2d\u7684Sirp\u03b1\u86cb\u767d\u3002<\/p>\n

d. \u901a\u8fc7RT-qPCR\u6d4b\u91cfWT BMDMs\uff08n = 3\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup> BMDMs\uff08n = 3\u4e2a\u6837\u672c\uff09\u4e2dSirpa\u3001Sirpb1\u548cSirpd\u7684\u8868\u8fbe\u6c34\u5e73\u3002<\/p>\n

e. \u6bd4\u8f83WT\u5c0f\u9f20\uff08n = 7\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 7\u4e2a\u6837\u672c\uff09\u80a0\u9053\u4e2d\u7684\u9ad3\u7cfb\u7ec6\u80de\u4e9a\u7fa4\u3002<\/p>\n

f. \u6bd4\u8f83WT\u5c0f\u9f20\uff08n = 7\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u4e2a\u6837\u672c\uff09\u80a0\u9053\u4e2d\u7684T\u7ec6\u80de\u4e9a\u7fa4\u3002<\/p>\n

g. \u6bd4\u8f83WT\u5c0f\u9f20\uff08n = 7\u4e2a\u6837\u672c\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 7\u62166\u4e2a\u6837\u672c\uff09\u4e2d\u6240\u6709\u80a0\u9053\u514d\u75ab\u7ec6\u80de\u4e2dNKT\u7ec6\u80de\u3001NK\u7ec6\u80de\u3001\u603b\u9ad3\u7cfb\u7ec6\u80de\u3001\u603bT\u7ec6\u80de\u548cB\u7ec6\u80de\u7684\u6bd4\u4f8b\u3002<\/p>\n

h-i. \u9ad3\u7cfb\u7ec6\u80de\uff08h\uff09\u6216\u6dcb\u5df4\u7ec6\u80de\uff08i\uff09\u7684\u95e8\u63a7\u7b56\u7565\u3002<\/p>\n

\u6240\u6709\u6837\u672c\u5747\u6765\u6e90\u4e8e\u72ec\u7acb\u7684\u5c0f\u9f20\u3002\u7ed3\u679c\uff08b, c\uff09\u4ee3\u8868\u4e09\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e2d\u7684\u4e00\u4e2a\u3002\u7ed3\u679c\uff08d-g\uff09\u6765\u81ea\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u8bef\u5dee\u6761\u8868\u793a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u5bf9d-g\u4f7f\u7528\u4e86\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n
\n

\u6269\u5c55\u6570\u636e\u56fe 4 | Sirp\u03b1\u7f3a\u5931\u6291\u5236\u80bf\u7624\u751f\u957f\uff0c\u4e14Sirp\u03b1\u4e0e\u60a3\u8005\u9884\u540e\u4e0d\u826f\u76f8\u5173\u3002<\/strong><\/p>\n

a. \u643a\u5e26\u76ae\u4e0bLLC\u80bf\u7624\u7684WT\u5c0f\u9f20\uff08n = 12\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 13\u53ea\uff09\u7684\u751f\u5b58\u5206\u6790\u3002<\/p>\n

b. \u643a\u5e26\u76ae\u4e0bHepa1-6\u80bf\u7624\u7684WT\u5c0f\u9f20\uff08n = 7\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 7\u53ea\uff09\u7684\u751f\u5b58\u5206\u6790\u3002<\/p>\n

c, e, g. \u7ed3\u80a0\u764c\uff08GSE17536\u548cGSE17537\uff0cn = 232\uff09\uff08c\uff09\u3001\u80ba\u9cde\u72b6\u7ec6\u80de\u764c\uff08TCGA-LUSC\uff0cn = 493\uff09\uff08e\uff09\u548c\u809d\u764c\uff08TCGA-LIHC\uff0cn = 365\uff09\uff08g\uff09\u4e2dSIRPA\u9ad8\u8868\u8fbe\u548c\u4f4e\u8868\u8fbe\u60a3\u8005\u7684\u603b\u751f\u5b58\u7387\u3002<\/p>\n

d, f, h. \u7ed3\u80a0\u764c\uff08GSE17536\u548cGSE17537\uff0cn = 187\uff09\uff08d\uff09\u3001\u80ba\u9cde\u72b6\u7ec6\u80de\u764c\uff08TCGA-LUSC\uff0cn = 476\uff09\uff08f\uff09\u548c\u809d\u764c\uff08TCGA-LIHC\uff0cn = 359\uff09\uff08h\uff09\u4e2dSIRPA\u9ad8\u8868\u8fbe\u548c\u4f4e\u8868\u8fbe\u60a3\u8005\u7684\u65e0\u75c5\u751f\u5b58\u7387\u3002<\/p>\n

i-j. DSS\u8bf1\u5bfc\u8fc7\u7a0b\u4e2dWT\u5c0f\u9f20\uff08n = 7\u53ea\uff09\u548cSirpa-\/-\u5c0f\u9f20\uff08n = 7\u53ea\uff09\u7684\u4f53\u91cd\u53d8\u5316\uff08i\uff09\u548c\u7ed3\u80a0\u708e\u75c5\u60c5\u6d3b\u52a8\u6307\u6570\uff08j\uff09\u3002\u75c5\u60c5\u6d3b\u52a8\u6307\u6570\u901a\u8fc7\u603b\u7ed3\u4f53\u91cd\u635f\u5931\u5206\u6570\u8ba1\u7b97\u5f97\u51fa\u3002<\/p>\n

k. MC38-Vector\u548cMC38-CD47\u2212\/\u2212<\/sup>\u7ec6\u80de\u5728\u57fa\u7840\u548c\u708e\u75c7\u6761\u4ef6\u4e0bCD47\u7684\u8868\u8fbe\u6c34\u5e73\uff08n = 3\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff09\u3002<\/p>\n

l. \u672a\u7ecf\u6cbb\u7597\u7684WT\u5c0f\u9f20\uff08\u5bf9\u7167\u7ec4\uff1bn = 6\u53ea\uff09\u3001\u63a5\u53d7\u6297CD47\u6cbb\u7597\u7684WT\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u3001\u63a5\u53d7Sirpa-ED-FC\u86cb\u767d\u6cbb\u7597\u7684WT\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u548c\u672a\u7ecf\u6cbb\u7597\u7684Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 9\u53ea\uff09\u4e2dMC38\u80bf\u7624\u7684\u751f\u957f\u60c5\u51b5\u3002<\/p>\n

\u6570\u636e\u6c47\u603b\u81ea2\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08a\uff09\u30013\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08k\uff09\u6216\u6765\u81ea5\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e2d\u7684\u4e00\u4e2a\u4ee3\u8868\uff08b\uff09\uff0c\u4ee5\u53ca\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\uff08i, j, l\uff09\u3002\u751f\u5b58\u5206\u6790\u7684P\u503c\uff08a-h\uff09\u901a\u8fc7\u5bf9\u6570\u79e9\u68c0\u9a8c\u83b7\u5f97\u3002\u4f53\u91cd\u548c\u75c5\u60c5\u6d3b\u52a8\u6307\u6570\u7684P\u503c\uff08i, j, l\uff09\u901a\u8fc7\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u83b7\u5f97\uff0ck\u7684P\u503c\u901a\u8fc7\u6bd4\u4f8b\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u83b7\u5f97\u3002\u8bef\u5dee\u6761\u8868\u793a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
<\/section>\n<\/section>\n
\n

\u6269\u5c55\u6570\u636e\u56fe 5 | Sirp\u03b1\u7f3a\u5931\u91cd\u5851\u80bf\u7624\u6d78\u6da6\u514d\u75ab\u7ec6\u80de\uff08TIICs\uff09\u7684\u7ec6\u80de\u6784\u6210\u548c\u514d\u75ab\u529f\u80fd\u3002<\/strong><\/p>\n

a. \u6240\u6709\u514d\u75ab\u7ec6\u80de\u7684t-SNE\u53ef\u89c6\u5316\uff0c\u6309\u91ce\u751f\u578b\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u7684\u6765\u6e90\u7740\u8272\u3002<\/p>\n

b. \u6240\u6709\u514d\u75ab\u7ec6\u80de\u4e2d\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u524d\u4e94\u4e2a\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u5e73\u5747\u8868\u8fbe\u91cf\u3002<\/p>\n

c. \u901a\u8fc7\u5355\u7ec6\u80de\u89e3\u5377\u79ef\uff08scDC\uff09\u8ba1\u7b97\u7684WT\u5c0f\u9f20\u548cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u4e2dAOM\/DSS\u8bf1\u5bfc\u7684\u80bf\u7624\u5185\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u6bd4\u4f8b\u3002P\u503c\u901a\u8fc7scDC\u4e2d\u7684GLM\u6a21\u578b\u7684\u81ea\u52a9\u91cd\u91c7\u6837\u8ba1\u7b97\u5f97\u51fa\u3002<\/p>\n

d. \u901a\u8fc7\u6d41\u5f0f\u7ec6\u80de\u672f\u5206\u6790\u7684WT\u5c0f\u9f20\uff08n = 9-12\u4e2a\u6837\u672c\uff09\u6216Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6-9\u4e2a\u6837\u672c\uff09\u4e2dAOM\/DSS\u8bf1\u5bfc\u7684\u81ea\u53d1\u6027\u7ed3\u76f4\u80a0\u80bf\u7624\u4e2d\u7684\u514d\u75ab\u7ec6\u80de\u767e\u5206\u6bd4\u3002<\/p>\n

e. WT\u5c0f\u9f20\u4e0e\u5176Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u5bf9\u5e94\u7269\u4e4b\u95f4AOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d\u6bcf\u79cd\u7ec6\u80de\u7c7b\u578b\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u6570\u91cf\u3002<\/p>\n

f. \u4f7f\u7528QuSAGE\u6bd4\u8f83Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u548cWT\u5c0f\u9f20\u4e2dAOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2dmMDSC\u7684\u6297\u539f\u5448\u9012\u8bc4\u5206\u3001\u541e\u566c\u8bc4\u5206\u548c\u708e\u75c7\u53cd\u5e94\u8bc4\u5206\u3002X\u8f74\u4ee3\u8868\u57fa\u56e0\u96c6\u8bc4\u5206\u7684log2\uff08\u500d\u6570\u53d8\u5316\uff09\u3002<\/p>\n

g-i. \u5728Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u76f8\u6bd4WT\u5c0f\u9f20\u4e2d\uff0cAOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2dILCs\uff08g\uff09\u3001B\u7ec6\u80de\uff08h\uff09\u548c\u6d46\u7ec6\u80de\uff08i\uff09\u7684\u663e\u8457\u5bcc\u96c6\u7279\u5f81\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

\u6240\u6709\u6837\u672c\u5747\u6765\u81ea\u72ec\u7acb\u7684\u5c0f\u9f20\u3002\u6570\u636e\uff08d\uff09\u8868\u793a\u4e3a\u6765\u81ea4\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u7684\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\uff0c\u5e76\u4f7f\u7528\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u8fdb\u884c\u5206\u6790\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
<\/section>\n<\/section>\n
\n

\u6269\u5c55\u6570\u636e\u56fe 6 | Sirp\u03b1\u7f3a\u5931\u91cd\u7f16\u7a0b\u4e86\u80bf\u7624\u4e2d\u7684\u5de8\u566c\u7ec6\u80de\u4e9a\u7fa4\u548c\u8c31\u7cfb\u3002<\/strong><\/p>\n

a. \u6240\u6709\u9ad3\u7cfb\u7ec6\u80de\u4e9a\u7fa4\u4e2d\u6bcf\u4e2a\u4e9a\u7fa4\u7684\u524d\u4e09\u4e2a\u7279\u5f02\u6027\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\u3002<\/p>\n

b. mMDSC1-2\u548cTAM1-4\u7c07\u5bcc\u96c6\u7684\u6700\u663e\u8457\u7279\u5f81\u3002<\/p>\n

c. \u5728\u62df\u65f6\u5e8f\u5206\u6790\u4e2d\uff0c\u6839\u636e\u5728\u4f2a\u65f6\u95f4\u4e2d\u7684\u5cf0\u503c\u6392\u5e8f\uff0c\u8868\u8fbe\u503c\u4e0e\u8c31\u7cfb1\uff08\u81f3TAM3\uff09\u547d\u8fd0\u6982\u7387\u6700\u4f73\u76f8\u5173\u7684\u524d100\u4e2a\u57fa\u56e0\u7684\u5e73\u6ed1\u5316\u8868\u8fbe\u8d8b\u52bf\u3002\u5e76\u975e\u6240\u6709\u57fa\u56e0\u540d\u79f0\u90fd\u663e\u793a\u51fa\u6765\u3002<\/p>\n

d. \u4f2a\u65f6\u95f4\u4e2d\u4e0e\u8c31\u7cfb1\uff08\u81f3TAM3\uff09\u76f8\u5173\u7684\u57fa\u56e0Dnase1l3\u3001Cd81\u3001S100a10\u548cTmsb10\u7684\u5e73\u6ed1\u5316\u8868\u8fbe\u8d8b\u52bf\uff0895%\u7f6e\u4fe1\u533a\u95f4\uff09\u3002<\/p>\n

e. \u4e0eTAM2\u76f8\u6bd4\uff0c\u5728TAM3\u4e2d\u8868\u8fbe\u4e0a\u8c03\u7684\u57fa\u56e0\uff08\u9876\u90e8\uff09\u548c\u8868\u8fbe\u4e0b\u8c03\u7684\u57fa\u56e0\uff08\u5e95\u90e8\uff09\u3002\u6240\u6709\u57fa\u56e0\u5728TAM3\u548cTAM2\u4e9a\u7fa4\u95f4\u663e\u793a\u51fa\u663e\u8457\u7684\u7edf\u8ba1\u5dee\u5f02\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff0cp < 0.05\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
<\/section>\n<\/section>\n
\n

\u6269\u5c55\u6570\u636e\u56fe 7 | Sirp\u03b1\u7f3a\u5931\u5728\u80bf\u7624\u4e2d\u91cd\u7f16\u7a0b\u4e86gMDSC\u4e9a\u7fa4\u548c\u8c31\u7cfb\u3002<\/strong><\/p>\n

a. gMDSC1-gMDSC8\u7c07\u7684\u663e\u8457\u5bcc\u96c6\u7279\u5f81\u3002<\/p>\n

b, c. \u5728\u62df\u65f6\u5e8f\u5206\u6790\u4e2d\uff0c\u6839\u636e\u5728\u4f2a\u65f6\u95f4\u4e2d\u7684\u5cf0\u503c\u6392\u5e8f\uff0c\u8868\u8fbe\u503c\u4e0e\u8c31\u7cfb1\uff08\u81f3gMDSC7\uff09\uff08b\uff09\u6216\u8c31\u7cfb2\uff08\u81f3gMDSC8\uff09\uff08c\uff09\u547d\u8fd0\u6982\u7387\u6700\u4f73\u76f8\u5173\u7684\u524d100\u4e2a\u57fa\u56e0\u7684\u5e73\u6ed1\u5316\u8868\u8fbe\u8d8b\u52bf\u3002\u5e76\u975e\u6240\u6709\u57fa\u56e0\u540d\u79f0\u90fd\u663e\u793a\u51fa\u6765\u3002<\/p>\n

d. \u62df\u65f6\u5e8f\u5206\u6790\u4e2d\u4e0e\u8c31\u7cfb1\uff08\u81f3gMDSC7\uff09\u548c\u8c31\u7cfb2\uff08\u81f3gMDSC8\uff09\u76f8\u5173\u7684\u57fa\u56e0Ifit3\u3001Rsad2\u3001Ftl1\u548cCstb\u7684\u5e73\u6ed1\u5316\u8868\u8fbe\u8d8b\u52bf\uff0895%\u7f6e\u4fe1\u533a\u95f4\uff09\u3002<\/p>\n

e. \u5728gMDSC6\/gMDSC7\u4e0egMDSC5\u6bd4\u8f83\u4e2d\uff0c\u8868\u8fbe\u4e0a\u8c03\u7684\u57fa\u56e0\uff08\u9876\u90e8\uff09\u548c\u8868\u8fbe\u4e0b\u8c03\u7684\u57fa\u56e0\uff08\u5e95\u90e8\uff09\u3002\u6240\u6709\u57fa\u56e0\u5728gMDSC6\/gMDSC7\u548cgMDSC5\u4e9a\u7fa4\u95f4\u663e\u793a\u51fa\u663e\u8457\u7684\u7edf\u8ba1\u5dee\u5f02\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff0cp < 0.05\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
<\/section>\n<\/section>\n
\n

\u6269\u5c55\u6570\u636e\u56fe 8 | Sirp\u03b1-\/-<\/sup> gMDSCs\u5728\u6297\u80bf\u7624\u514d\u75ab\u548cTAMs\u4e2d\u7684\u4f5c\u7528\u8f83\u5f31\u3002<\/strong><\/p>\n

a. WT\u5c0f\u9f20\u4e2dTIICs\u7684Sirpa\u7684t-SNE\u53ef\u89c6\u5316\uff0c\u6839\u636e\u8868\u8fbe\u6c34\u5e73\u7740\u8272\u3002<\/p>\n

b. \u5c0f\u9f20\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u6bcf\u79cd\u514d\u75ab\u7ec6\u80de\u7c7b\u578b\u4e2dSirpa\u7684\u8868\u8fbe\u3002\u4e2d\u95f4\u7684\u767d\u70b9\u548c\u7ebf\u4ee3\u8868\u4e2d\u4f4d\u6570\u548c\u56db\u5206\u4f4d\u6570\u503c\u3002<\/p>\n

c. WT\u5c0f\u9f20\uff08n = 9\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u3001WT + \u03b1Ly6G\u5c0f\u9f20\uff08n = 7\u53ea\uff09\u548cSirpa\u2212\/\u2212<\/sup> + \u03b1Ly6G\u5c0f\u9f20\u7ec4\uff08n = 5\u53ea\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u3002<\/p>\n

d. WT\u5c0f\u9f20\uff08n = 5\u53ea\uff09\u3001Sirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\uff08n = 5\u53ea\uff09\u3001WT + WT-MDSCs\u5c0f\u9f20\uff08n = 6\u53ea\uff09\u548cWT + Sirpa\u2212\/\u2212<\/sup>MDSCs\u5c0f\u9f20\u7ec4\uff08n = 7\u53ea\uff09\u4f53\u5185\u76ae\u4e0bMC38\u80bf\u7624\u7684\u751f\u957f\u3002<\/p>\n

e. \u5728MC38\u6a21\u578b\u4e2d\uff0cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u548cWT\u5c0f\u9f20\u4e4b\u95f4TAMs\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u5bcc\u96c6\u7279\u5f81\uff08\u6279\u91cfRNA\u6d4b\u5e8f\u6570\u636e\uff09\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

f. \u5728MC38\u6a21\u578b\u4e2d\uff0cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u548cWT\u5c0f\u9f20\u4e4b\u95f4TAMs\u7684\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u70ed\u56fe\uff0c\u4e3b\u8981\u663e\u793a\u4e86\u4e0e\u8d8b\u5316\u56e0\u5b50\u3001\u708e\u75c7\u53cd\u5e94\u3001\u541e\u566c\u4f5c\u7528\u548c\u6297\u539f\u5448\u9012\u76f8\u5173\u7684\u57fa\u56e0\uff08\u6279\u91cfRNA\u6d4b\u5e8f\u6570\u636e\uff09\u3002<\/p>\n

g. \u5728MC38\u6a21\u578b\uff08\u6279\u91cfRNA\u6d4b\u5e8f\u6570\u636e\uff09\u548cAOM\/DSS\u6a21\u578b\uff08\u5355\u7ec6\u80deRNA\u6d4b\u5e8f\u6570\u636e\uff09\u4e2d\uff0cSirpa\u2212\/\u2212<\/sup>\u5c0f\u9f20\u9ad8\u8868\u8fbe\u57fa\u56e0\u5728TAMs\u4e2d\u7684\u5bcc\u96c6\u7279\u5f81\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

\u7ed3\u679c\uff08c\uff09\u4ee3\u8868\u4e09\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u4e2d\u7684\u4e00\u4e2a\u3002\u7ed3\u679c\uff08d\uff09\u6765\u81ea\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u8bef\u5dee\u6761\u663e\u793a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u5bf9\u4e8ec\u548cd\uff0c\u4f7f\u7528\u4e86\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6269\u5c55\u6570\u636e\u56fe 9 | Sirpa-\/-<\/sup>\u9ad3\u7cfb\u7ec6\u80de\u589e\u5f3a\u4e86T\u6dcb\u5df4\u7ec6\u80de\u7684\u6fc0\u6d3b\u3002<\/strong><\/p>\n

a, b. \u5728AOM\/DSS\u8bf1\u5bfc\u7684CRC\u4e2d\uff0cWT\u5c0f\u9f20\u4e0eSirpa-\/-<\/sup>\u5c0f\u9f20\u4e4b\u95f4CD8+ <\/sup>T\u7ec6\u80de\uff08a\uff09\u6216CD4+ <\/sup>T\u7ec6\u80de\uff08b\uff09\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u8868\u8fbe\uff08Wilcoxon\u68c0\u9a8c\uff0c\u53cc\u4fa7\uff09\u3002<\/p>\n

c-d. \u5728AOM\/DSS\u8bf1\u5bfc\u7684WT\u5c0f\u9f20\u548cSirpa-\/-<\/sup>\u5c0f\u9f20\u4e2d\uff0c\u7ecfCD4+<\/sup>\u6216CD8+<\/sup>\u6297\u4f53\u5904\u7406\u540e\u6d4b\u91cf\u7684\u80bf\u7624\u8d1f\u8377\uff08c\uff09\u548c\u80bf\u7624\u6570\u91cf\uff08d\uff09\uff0cWT\uff08n = 9\u53ea\u5c0f\u9f20\uff09\uff0cSirpa-\/-<\/sup>\uff08n = 6\u53ea\u5c0f\u9f20\uff09\uff0cWT + \u03b1CD4\uff08n = 5\u53ea\u5c0f\u9f20\uff09\uff0cSirpa-\/-<\/sup> + \u03b1CD4\uff08n = 3\u53ea\u5c0f\u9f20\uff09\uff0cWT + \u03b1CD8\uff08n = 3\u53ea\u5c0f\u9f20\uff09\uff0cSirpa-\/-<\/sup> + \u03b1CD8\uff08n = 4\u53ea\u5c0f\u9f20\uff09\u3002<\/p>\n

e. \u901a\u8fc7CellChat\u8ba1\u7b97\u7684AOM\/DSS\u5904\u7406\u7684WT\u5c0f\u9f20\u548cSirpa-\/-<\/sup>\u5c0f\u9f20\u4e2dTAM\u3001mMDSC\u3001cDC1\u548ccDC2\uff08\u53d1\u9001\u8005\uff09\u5230\u5176\u4ed6\u7ec6\u80de\u7c7b\u578b\uff08\u63a5\u6536\u8005\uff09\u7684\u7ec6\u80de-\u7ec6\u80de\u63a5\u89e6\u4fe1\u53f7\u4f20\u9012\u7684L\u2013R\u5bf9\u3002\u7ebf\u5bbd\u8868\u793a\u4fe1\u53f7\u5f3a\u5ea6\uff0c\u7ebf\u989c\u8272\u4e0e\u4fe1\u53f7\u53d1\u9001\u8005\u76f8\u540c\u3002<\/p>\n

f. \u901a\u8fc7CellChat\u8ba1\u7b97\u7684\u7ec6\u80de-\u7ec6\u80de\u63a5\u89e6\u4fe1\u53f7\u7f51\u7edc\u4e2d\u7684\u4e3b\u8981\u53d1\u9001\u8005\u548c\u63a5\u6536\u8005\u7ec6\u80de\u7c7b\u578b\u3002\u8ba1\u6570\u4ee3\u8868L\u2013R\u5bf9\u7684\u6570\u91cf\u3002<\/p>\n

g. \u4e0d\u540c\u6bd4\u4f8b\u4e0b\uff0cWT\uff08n = 3\u4e2a\u6837\u672c\uff09\u6216Sirpa-\/-<\/sup>\uff08n = 3\u4e2a\u6837\u672c\uff09gMDSCs\u6291\u5236\u7684CD8+ T\u7ec6\u80de\u589e\u6b96\u3002\u6240\u6709\u6837\u672c\u5747\u6765\u6e90\u4e8e\u72ec\u7acb\u5c0f\u9f20\u3002\u7ed3\u679c\uff08c, d, g\uff09\u6765\u81ea\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u8bef\u5dee\u6761\u8868\u793a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u5bf9c, d, g\u4f7f\u7528\u4e86\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6269\u5c55\u6570\u636e\u56fe 10 | Sirpa-\/-<\/sup>\u9ad3\u7cfb\u7ec6\u80de\u589e\u5f3a\u4e86T\u6dcb\u5df4\u7ec6\u80de\u7684\u62db\u52df\u3002<\/strong><\/p>\n

a. \u901a\u8fc7CellChat\u8ba1\u7b97\u7684AOM\/DSS\u5904\u7406\u7684WT\u5c0f\u9f20\u548cSirpa-\/-<\/sup>\u5c0f\u9f20\u4e2dmMDSC\u3001TAM\u548cgMDSC\uff08\u53d1\u9001\u8005\uff09\u5230\u5176\u4ed6\u7ec6\u80de\u7c7b\u578b\uff08\u63a5\u6536\u8005\uff09\u7684\u5206\u6ccc\u4fe1\u53f7\u4f20\u9012\u7684L\u2013R\u5bf9\u3002\u7ebf\u5bbd\u8868\u793a\u4fe1\u53f7\u5f3a\u5ea6\uff0c\u7ebf\u989c\u8272\u4e0e\u4fe1\u53f7\u53d1\u9001\u8005\u76f8\u540c\u3002<\/p>\n

b. \u901a\u8fc7CellChat\u8ba1\u7b97\u7684\u8d8b\u5316\u56e0\u5b50\u4fe1\u53f7\u901a\u8def\uff08CCL\u548cCXCL\u901a\u8def\uff09\u4e2d\u7684\u4e3b\u8981\u53d1\u9001\u8005\u548c\u63a5\u6536\u8005\u7ec6\u80de\u7c7b\u578b\u3002\u8ba1\u6570\u4ee3\u8868L\u2013R\u5bf9\u7684\u6570\u91cf\u3002<\/p>\n

c. WT\u5c0f\u9f20\uff08n = 4\u4e2a\u6837\u672c\uff09\u548cSirpa-\/-<\/sup>\u5c0f\u9f20\uff08n = 5\u4e2a\u6837\u672c\uff09MC38\u80bf\u7624\u4e2dTAMs\u7684\u5404\u79cd\u8d8b\u5316\u56e0\u5b50\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002<\/p>\n

d. \u76d2\u5f62\u56fe\u663e\u793a\u590d\u53d1\u6027\u80f6\u8d28\u6bcd\u7ec6\u80de\u7624\uff08GBM\uff09\u60a3\u8005\u5728\u65b0\u8f85\u52a9PD-1\u963b\u65ad\u6cbb\u7597\u524d\u540e\u5de8\u566c\u7ec6\u80de\u4e0aSIRPA\u7684\u8868\u8fbe\u3002<\/p>\n

\u6240\u6709\u6837\u672c\u5747\u6765\u6e90\u4e8e\u72ec\u7acb\u5c0f\u9f20\u3002\u4f7f\u7528\u53cc\u4fa7\u672a\u914d\u5bf9t\u68c0\u9a8c\u8ba1\u7b97P\u503c\u3002\u7ed3\u679c\uff08c\uff09\u6765\u81ea\u4e00\u4e2a\u72ec\u7acb\u5b9e\u9a8c\u3002\u8bef\u5dee\u6761\u8868\u793a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u5bf9c\u4f7f\u7528\u4e86\u672a\u914d\u5bf9\u7684\u5b66\u751ft\u68c0\u9a8c\uff08\u53cc\u5c3e\uff09\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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OncoLab\u516c\u4f17\u53f7\u5df2\u589e\u52a0\u671f\u520a\u67e5\u8be2\u529f\u80fd<\/span><\/strong>\uff0cSCI\u671f\u520a<\/strong><\/span>\u53ef\u67e5\u8be2\u671f\u520a\u540d\u79f0\u7f29\u5199\u3001ISSN\u520a\u53f7\u3001\u8fd1\u4e94\u5e74\u5f71\u54cd\u56e0\u5b50<\/strong>\u3001\u6700\u65b0WOS\u7684JCR\u5206\u533a<\/strong>\u3001\u6700\u65b0\u4e2d\u79d1\u9662\u5206\u533a<\/strong>\u4ee5\u53ca\u4e2d\u79d1\u9662\u671f\u520a\u9884\u8b66\u4fe1\u606f<\/strong>\uff1b<\/p>\n

\u4e2d\u6587\u671f\u520a<\/span><\/strong>\u53ef\u68c0\u7d22CSCD\u6838\u5fc3<\/strong>\u3001\u5317\u5927\u4e2d\u6587\u6838\u5fc3<\/strong>\u3001\u79d1\u6280\u6838\u5fc3<\/strong>\u6536\u5f55\u60c5\u51b5\uff1b<\/p>\n

\u5728\u516c\u4f17\u53f7\u5bf9\u8bdd\u6846\u53d1\u9001\u6742\u5fd7\u540d\u79f0<\/span><\/strong>\u5373\u53ef\u5feb\u901f\u68c0\u7d22\uff0c\u652f\u6301\u6a21\u7cca\u68c0\u7d22\uff0c\u6b22\u8fce\u4f7f\u7528\uff01<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\u2014 THE  END \u2014<\/span><\/section>\n
\u6765\u6e90 | Nature Cancer<\/span><\/section>\n
\u7b5b\u9009\u6574\u7406 | \u738b\u5764<\/span><\/span><\/span><\/section>\n
\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u53ef\u8f6c\u5230\u6587\u7ae0\u9875\u9762
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\u516c\u4f17\u53f7\u53d1\u90012024011006\u53ef\u83b7\u53d6\u6587\u732e\u5168\u6587<\/span><\/span><\/span><\/section>\n


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\u2589  \u5f80\u671f\u63a8\u9001\u5408\u8f91<\/strong><\/strong><\/section>\n
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\u80bf\u7624\u514d\u75ab\u5b66\u6700\u65b0\u6587\u732e<\/span><\/a>
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\u514d\u75ab\u5b66\u62fe\u9057\u7cfb\u5217<\/a><\/span><\/p>\n

\u80bf\u7624\u514d\u75ab\u5b66\u7cfb\u5217<\/span><\/a><\/p>\n

ACIR\u6587\u732e\u901f\u9012<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u4f5c\u56fe<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u5199\u4f5c\u53ca\u6295\u7a3f<\/span><\/a><\/p>\n

\u751f\u4fe1\u5165\u95e8\u5b66\u4e60\u7b14\u8bb0<\/span><\/a><\/p>\n

R\u8bed\u8a00\u57fa\u7840\u4e0e\u5355\u7ec6\u80de\u5206\u6790\u5b9e\u6218\u89c6\u9891\u8bfe\u7a0b<\/span><\/a><\/p>\n

\u751f\u7269\u5b66\u5b9e\u9a8c\u6280\u672f\u53ca\u5e38\u7528\u8f6f\u4ef6<\/span><\/a><\/p>\n

RCIM\u4eea\u5668\u7b80\u4ecb\u53ca\u64cd\u4f5c\u89c6\u9891<\/a><\/span><\/p>\n

\u5e38\u7528\u7535\u8111\u8f6f\u4ef6\u4f7f\u7528\u6559\u7a0b<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u52a0\u5165\u8bfb\u8005\u4ea4\u6d41\u7fa4~<\/span><\/p>\n

\uff08\u6dfb\u52a0\u8bf7\u5907\u6ce8\u5355\u4f4d\u59d3\u540d\uff09<\/span><\/p>\n


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\u52a0\u5165\u77e5\u8bc6\u661f\u7403~<\/span><\/p>\n


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\u70b9\u4eae\u8d5e\u4e0e\u5728\u770b <\/span><\/p>\n

\u8ba9\u66f4\u591a\u4eba\u770b\u5230<\/span><\/p>\n

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\u672c\u7bc7\u6587\u7ae0\u6765\u6e90\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7: OncoLab <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u6742\u5fd7\u540d\u79f0\uff1aNat Cancer. \u53d1\u8868\u65e5\u671f\uff1a2024.01.10 DOI\uff1a10.1038\/s43018-023…<\/p>\n","protected":false},"author":1,"featured_media":3391,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[63],"tags":[],"_links":{"self":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/3409"}],"collection":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/comments?post=3409"}],"version-history":[{"count":0,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/3409\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/media\/3391"}],"wp:attachment":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/media?parent=3409"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/categories?post=3409"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/tags?post=3409"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}