{"id":3443,"date":"2024-04-07T07:03:43","date_gmt":"2024-04-06T23:03:43","guid":{"rendered":"http:\/\/oncolab.cn\/index.php\/2024\/04\/07\/cancer-cell%ef%bc%9a%e7%bb%b4%e7%94%b2%e9%85%b8%e5%8f%97%e4%bd%93%ef%bc%88rar%ef%bc%89%e6%bf%80%e6%b4%bb%e9%87%8d%e7%bc%96%e7%a8%8b%e7%bb%86%e8%83%9e%e8%a1%b0%e8%80%81%e5%8f%8d%e5%ba%94%e5%b9%b6\/"},"modified":"2024-04-07T07:03:43","modified_gmt":"2024-04-06T23:03:43","slug":"cancer-cell%ef%bc%9a%e7%bb%b4%e7%94%b2%e9%85%b8%e5%8f%97%e4%bd%93%ef%bc%88rar%ef%bc%89%e6%bf%80%e6%b4%bb%e9%87%8d%e7%bc%96%e7%a8%8b%e7%bb%86%e8%83%9e%e8%a1%b0%e8%80%81%e5%8f%8d%e5%ba%94%e5%b9%b6","status":"publish","type":"post","link":"http:\/\/oncolab.cn\/index.php\/2024\/04\/07\/cancer-cell%ef%bc%9a%e7%bb%b4%e7%94%b2%e9%85%b8%e5%8f%97%e4%bd%93%ef%bc%88rar%ef%bc%89%e6%bf%80%e6%b4%bb%e9%87%8d%e7%bc%96%e7%a8%8b%e7%bb%86%e8%83%9e%e8%a1%b0%e8%80%81%e5%8f%8d%e5%ba%94%e5%b9%b6\/","title":{"rendered":"Cancer Cell\uff1a\u7ef4\u7532\u9178\u53d7\u4f53\uff08RAR\uff09\u6fc0\u6d3b\u91cd\u7f16\u7a0b\u7ec6\u80de\u8870\u8001\u53cd\u5e94\u5e76\u589e\u5f3a\u81ea\u7136\u6740\u4f24\u7ec6\u80de\u7684\u6297\u80bf\u7624\u6d3b\u6027"},"content":{"rendered":"
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\u524d\u671f\u7cbe\u5f69\u5185\u5bb9\uff1a<\/span><\/p>\n

Nat Commun\uff1a\u8870\u8001\u901a\u8fc7\u8bf1\u5bfc\u514d\u75ab\u6291\u5236\u6027\u80bf\u7624\u5fae\u73af\u5883\u5bfc\u81f4\u514d\u75ab\u6cbb\u7597\u6297\u6027<\/span><\/a><\/p>\n

Cancer Cell\uff1a\u901a\u8fc7mRNA\u6280\u672f\u7f16\u7801\u6301\u4e45\u6027IL-2\u5728MHC I\u7c7b\u5206\u5b50\u7f3a\u9677\u764c\u75c7\u4e2d\u53ef\u6062\u590d\u9488\u5bf9\u65b0\u6297\u539f\u7684CD8+ T\u7ec6\u80de\u53cd\u5e94<\/span><\/a><\/p>\n

Cell Metab\uff1a\u996e\u98df\u4e2d\u7684\u53cd\u5f0f\u6cb9\u9178\u901a\u8fc7ACSL5\u589e\u5f3a\u80bf\u7624\u6297\u539f\u5448\u9012\u548c\u6297\u80bf\u7624\u514d\u75ab<\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6742\u5fd7\u540d\u79f0<\/strong>\uff1aCancer Cell<\/span><\/p>\n

\u53d1\u8868\u65e5\u671f<\/strong>\uff1a2024.02.27<\/p>\n

DOI<\/strong>\uff1a10.1016\/j.ccell.2024.02.004.<\/p>\n

PubMed<\/strong>\uff1ahttps:\/\/pubmed.ncbi.nlm.nih.gov\/38428412\/<\/p>\n

\u5f71\u54cd\u56e0\u5b50<\/strong>\uff1a50.3<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u5185\u5bb9\u7b80\u4ecb<\/strong><\/p>\n<\/section>\n

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\u7ec6\u80de\u8870\u8001\u5728\u80bf\u7624\u4e2d\u53ef\u4ee5\u4ea7\u751f\u53cc\u91cd\u6548\u5e94\uff0c\u65e2\u53ef\u4ee5\u6291\u5236\u4e5f\u53ef\u4ee5\u4fc3\u8fdb\u80bf\u7624\u8fdb\u5c55\u3002\u7531\u8870\u8001\u7ec6\u80de\u91ca\u653e\u7684\u8870\u8001\u76f8\u5173\u5206\u6ccc\u8868\u578b\uff08SASP\uff09\u5728\u8fd9\u4e00\u4e8c\u5206\u6027\u4e2d\u53d1\u6325\u5173\u952e\u4f5c\u7528\u3002\u56e0\u6b64\uff0c\u4e34\u5e8a\u4e0a\u7684\u6311\u6218\u5728\u4e8e\u5f00\u53d1\u5b89\u5168\u589e\u5f3a\u764c\u75c7\u4e2d\u8870\u8001\uff0c\u5e76\u4e14\u504f\u597d\u4fc3\u8fdb\u6291\u7624\u7684SASP\u56e0\u5b50\u800c\u975e\u4fc3\u8fdb\u80bf\u7624\u7684\u56e0\u5b50\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u5728\u8fd9\u91cc\uff0c\u6211\u4eec\u786e\u5b9a\u4e86\u7ef4\u7532\u9178\u53d7\u4f53\uff08RAR\uff09\u6fc0\u52a8\u5242\u963f\u8fbe\u5e15\u6797\u4f5c\u4e3a\u524d\u5217\u817a\u764c\uff08PCa\uff09\u4e2d\u6709\u6548\u7684\u4fc3\u8870\u8001\u5316\u5408\u7269\u3002RAR\u7684\u91cd\u65b0\u6fc0\u6d3b\u89e6\u53d1\u4e86\u5f3a\u70c8\u7684\u8870\u8001\u53cd\u5e94\u548c\u6291\u7624SASP\u3002\u5728\u524d\u5217\u817a\u764c\u7684\u4e34\u5e8a\u524d\u5c0f\u9f20\u6a21\u578b\u4e2d\uff0c\u963f\u8fbe\u5e15\u6797\u4e0e\u591a\u897f\u4ed6\u8d5b\u7684\u8054\u5408\u4f7f\u7528\u4fc3\u8fdb\u4e86\u6291\u7624SASP\uff0c\u6bd4\u5355\u72ec\u4f7f\u7528\u4efb\u4e00\u836f\u7269\u90fd\u80fd\u66f4\u6709\u6548\u5730\u589e\u5f3a\u81ea\u7136\u6740\u4f24\uff08NK\uff09\u7ec6\u80de\u4ecb\u5bfc\u7684\u80bf\u7624\u6e05\u9664\u3002\u8fd9\u79cd\u65b9\u6cd5\u589e\u5f3a\u4e86\u4eba\u7c7bNK\u7ec6\u80de\u5bf9\u6ce8\u5c04\u4eba\u7c7b\u524d\u5217\u817a\u764c\u7ec6\u80de\u7684\u5c0f\u9f20\u540c\u79cd\u5f02\u4f53\u80bf\u7624\u7684\u7597\u6548\uff0c\u63d0\u793a\u4e86\u4e00\u79cd\u5728\u201c\u514d\u75ab\u8352\u6f20\u201d\u80bf\u7624\u4e2d\u523a\u6fc0\u6297\u80bf\u7624\u514d\u75ab\u53cd\u5e94\u7684\u66ff\u4ee3\u6cbb\u7597\u7b56\u7565\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u521b\u65b0\u6027\u53ca\u7814\u7a76\u610f\u4e49<\/strong><\/p>\n<\/section>\n

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\u8fd9\u7bc7\u6587\u7ae0\u7684\u6838\u5fc3\u521b\u65b0\u6027\u5728\u4e8e\u53d1\u73b0\u7ef4\u7532\u9178\u53d7\u4f53\uff08RAR\uff09\u6fc0\u52a8\u5242\u963f\u8fbe\u5e15\u6797\u5728\u524d\u5217\u817a\u764c\u4e2d\u4f5c\u4e3a\u6709\u6548\u7684\u4fc3\u8fdb\u8870\u8001\u5316\u5408\u7269\u3002\u7814\u7a76\u8868\u660e\uff0c\u901a\u8fc7\u91cd\u65b0\u6fc0\u6d3bRAR\uff0c\u53ef\u4ee5\u89e6\u53d1\u5f3a\u70c8\u7684\u7ec6\u80de\u8870\u8001\u53cd\u5e94\u548c\u5177\u6709\u6291\u5236\u80bf\u7624\u7279\u6027\u7684\u8870\u8001\u76f8\u5173\u5206\u6ccc\u578b\u8868\u578b\uff08SASP\uff09\u3002\u5728\u524d\u5217\u817a\u764c\u7684\u4e34\u5e8a\u524d\u5c0f\u9f20\u6a21\u578b\u4e2d\uff0c\u963f\u8fbe\u5e15\u6797\u4e0e\u591a\u897f\u4ed6\u8d5b\u7684\u7ec4\u5408\u80fd\u591f\u4fc3\u8fdb\u4e00\u79cd\u6291\u5236\u80bf\u7624\u7684SASP\uff0c\u8fd9\u79cd\u7ec4\u5408\u6bd4\u4efb\u4e00\u5355\u72ec\u836f\u7269\u66f4\u6709\u6548\u5730\u589e\u5f3a\u4e86\u81ea\u7136\u6740\u4f24\uff08NK\uff09\u7ec6\u80de\u4ecb\u5bfc\u7684\u80bf\u7624\u6e05\u9664\u3002<\/p>\n

\u8fd9\u9879\u7814\u7a76\u7684\u610f\u4e49\u5728\u4e8e\u63d0\u4f9b\u4e86\u4e00\u79cd\u6f5c\u5728\u7684\u6cbb\u7597\u7b56\u7565\uff0c\u5373\u901a\u8fc7\u6fc0\u6d3bRAR\u6765\u4fc3\u8fdb\u80bf\u7624\u5185\u7684\u8870\u8001\u53cd\u5e94\u548c\u6297\u80bf\u7624\u514d\u75ab\u53cd\u5e94\uff0c\u5c24\u5176\u662f\u5728\u90a3\u4e9b\u201c\u514d\u75ab\u5b66\u4e0a\u51b7\u6f20\u201d\u7684\u80bf\u7624\u4e2d\u3002\u901a\u8fc7\u8fd9\u79cd\u65b9\u5f0f\uff0c\u53ef\u4ee5\u589e\u5f3a\u4eba\u4f53NK\u7ec6\u80de\u5728\u5c0f\u9f20\u4f53\u5185\u5bf9\u4eba\u7c7b\u524d\u5217\u817a\u764c\u7ec6\u80de\u7684\u6740\u4f24\u6548\u679c\uff0c\u4e3a\u5f00\u53d1\u65b0\u7684\u6297\u80bf\u7624\u7597\u6cd5\u63d0\u4f9b\u4e86\u91cd\u8981\u7684\u5b9e\u9a8c\u4f9d\u636e\u3002\u8fd9\u4e0d\u4ec5\u4e3a\u80bf\u7624\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u89c6\u89d2\uff0c\u4e5f\u4e3a\u7406\u89e3\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u7684\u7ec6\u80de\u8870\u8001\u673a\u5236\u548c\u514d\u75ab\u53cd\u5e94\u63d0\u4f9b\u4e86\u66f4\u6df1\u5165\u7684\u6d1e\u5bdf\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6570\u636e\u56fe<\/strong><\/p>\n<\/section>\n

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\u56fe 1. \u53d1\u73b0RAR\u6fc0\u52a8\u5242\u4e3a\u4fc3\u8fdb\u8870\u8001\u7684\u5316\u5408\u7269 <\/strong><\/p>\n

(A) \u7279\u5b9a\u5316\u5408\u7269\u5904\u7406\u540ePC3\u7ec6\u80de\u589e\u6b96\u500d\u6570\u7684\u53d8\u5316\uff0c\u4ee5\u65f6\u95f40\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\u3002  <\/p>\n

(B) \u7279\u5b9a\u5316\u5408\u7269\u5904\u7406\u540ePC3shTIMP1\u7ec6\u80de\u589e\u6b96\u500d\u6570\u7684\u53d8\u5316\uff0c\u4ee5\u65f6\u95f40\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\u3002  <\/p>\n

(C) \u7279\u5b9a\u5316\u5408\u7269\u5904\u7406\u540eLNCaP\u7ec6\u80de\u589e\u6b96\u500d\u6570\u7684\u53d8\u5316\uff0c\u4ee5\u65f6\u95f40\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\u3002  <\/p>\n

(D) \u7279\u5b9a\u5316\u5408\u7269\u5904\u7406\u540e22RV1\u7ec6\u80de\u589e\u6b96\u500d\u6570\u7684\u53d8\u5316\uff0c\u4ee5\u65f6\u95f40\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\u3002  <\/p>\n

(E) \u7ecf\u8fc7\u5904\u7406\u7684PC3\u7ec6\u80de\u4e2dSA-\u03b2 Gal\u7684\u5b9a\u91cf\u3002  <\/p>\n

(F) \u7ecf\u8fc7\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u4e2dSA-\u03b2 Gal\u7684\u5b9a\u91cf\u3002  <\/p>\n

(G) \u7ecf\u8fc7\u5904\u7406\u7684LNCaP\u7ec6\u80de\u4e2dSA-\u03b2 Gal\u7684\u5b9a\u91cf\u3002  <\/p>\n

(H) \u7ecf\u8fc7\u5904\u7406\u768422RV1\u7ec6\u80de\u4e2dSA-\u03b2 Gal\u7684\u5b9a\u91cf\u3002  <\/p>\n

(I) PC3\u7ec6\u80de\u4e2d\u8870\u8001\u6807\u5fd7\u7269\u7684RT-qPCR\u5206\u6790\u3002  <\/p>\n

(J) PC3shTIMP1\u7ec6\u80de\u4e2d\u8870\u8001\u6807\u5fd7\u7269\u7684RT-qPCR\u5206\u6790\u3002  <\/p>\n

(K) LNCaP\u7ec6\u80de\u4e2d\u8870\u8001\u6807\u5fd7\u7269\u7684RT-qPCR\u5206\u6790\u3002  <\/p>\n

(L) 22RV1\u7ec6\u80de\u4e2d\u8870\u8001\u6807\u5fd7\u7269\u7684RT-qPCR\u5206\u6790\u3002  <\/p>\n

(M) \u5b9e\u9a8c\u8bbe\u8ba1\uff08\u4e0a\u56fe\uff09\u53ca\u7ecf\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u7684\u514d\u75ab\u5370\u8ff9\u5b9a\u91cf\uff08\u4e0b\u56fe\uff09\uff0c\u53c2\u89c1\u56feS1J\u3002  <\/p>\n

(N) \u7ecfp21\u6291\u5236\u5242\uff08p21i\uff09UC2288\u548cadapalene\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u4e2dSA-\u03b2 Gal\u7684\u5b9a\u91cf\u3002  <\/p>\n

(O) \u7ecfp21\u6291\u5236\u5242\uff08p21i\uff09UC2288\u548cadapalene\u5904\u7406\u540ePC3shTIMP1\u7ec6\u80de\u589e\u6b96\u500d\u6570\u7684\u53d8\u5316\uff0c\u4ee5\u65f6\u95f40\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\uff08\u5de6\u56fe\uff09\uff0c\u4ee5\u53ca\u7ed3\u6676\u7d2b\u67d3\u8272\u5b9a\u91cf\uff08\u53f3\u56fe\uff09\u3002<\/p>\n

\u6570\u636e(E\u2013N)\u548c(O\u53f3\u56fe)\u8868\u793a\u4e3a\u5747\u503c \u00b1 \u6807\u51c6\u5dee\uff1b\u6570\u636e(A\u2013D)\u548c(O\u5de6\u56fe)\u8868\u793a\u4e3a\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u3002\u6570\u636e\u4ee3\u8868\u4e09\u5230\u4e94\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002\u6570\u636e(M)\u4ee3\u8868\u4e00\u6b21\u5b9e\u9a8c\u3002\u6570\u636e(E\u2013H)\u7684\u7edf\u8ba1\u6d4b\u8bd5\u4f7f\u7528\u5355\u5411\u65b9\u5dee\u5206\u6790\u540e\u63a5Tukey\u2019s\u6d4b\u8bd5 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e(A\u2013D)\u548c(I\u2013O)\u7684\u7edf\u8ba1\u6d4b\u8bd5\u4f7f\u7528\u53cc\u5411\u65b9\u5dee\u5206\u6790 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u53e6\u89c1\u56feS1\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 2. \u7ef4\u7532\u9178\u4ee3\u8c22\u548c\u901a\u8def\u5728\u524d\u5217\u817a\u764c\u53d1\u5c55\u8fc7\u7a0b\u4e2d\u53d7\u635f<\/strong><\/p>\n

(A) \u7ef4\u6069\u56fe\u5c55\u793a\u4e86\u6bcf\u4e2a\u4e0eRA\u76f8\u5173\u7684\u7279\u5f81\u4e2d\u5dee\u5f02\u8868\u8fbe\u57fa\u56e0\u7684\u6570\u91cf\u3002  <\/p>\n

(B) \u5229\u7528\u6307\u5b9a\u7814\u7a76\u83b7\u5f97\u7684\u5168\u524d\u5217\u817a\u764c\u8f6c\u5f55\u7ec4\u8fdb\u884c\u4e3b\u6210\u5206\u5206\u6790\uff08PCA\uff09\uff0c\u6bd4\u8f83\u6b63\u5e38\u524d\u5217\u817a\uff08\u7eff\u8272\uff09\u3001\u539f\u53d1\u6027\u524d\u5217\u817a\u764c\uff08\u7d2b\u8272\uff09\u3001\u53bb\u52bf\u62b5\u6297\u6027\u524d\u5217\u817a\u764c\uff08CRPC\uff0c\u6a59\u8272\uff09\u548c\u795e\u7ecf\u5185\u5206\u6ccc\u524d\u5217\u817a\u764c\uff08NEPC\uff0c\u9ec4\u8272\uff09\u3002  <\/p>\n

(C) PCA\u5c55\u793a\u4e86PCa\u75be\u75c5\u8fdb\u5c55\u8fc7\u7a0b\u4e2dRAR\u4fe1\u53f7\u901a\u8def\u7684\u6fc0\u6d3b\u60c5\u51b5\u3002  <\/p>\n

(D) PCA\u5c55\u793a\u4e86PCa\u75be\u75c5\u8fdb\u5c55\u8fc7\u7a0b\u4e2dRAR\u4e0b\u6e38\u76ee\u6807\u7684\u8868\u8fbe\u60c5\u51b5\u3002  <\/p>\n

(E) PCA\u5c55\u793a\u4e86PCa\u75be\u75c5\u8fdb\u5c55\u8fc7\u7a0b\u4e2dRA\u4ee3\u8c22\u76f8\u5173\u57fa\u56e0\u7684\u8868\u8fbe\u60c5\u51b5\u3002  <\/p>\n

(F) \u56fe\u8868\u5c55\u793a\u4e86\u60a3\u8005RNA-seq\u6570\u636e\u4e2dRA\u76f8\u5173\u57fa\u56e0\u7684\u76ae\u5c14\u68ee\u76f8\u5173\u6027\u3002\u7070\u8272\u70b9\u4ee3\u8868\u4e0eRAR\u901a\u8def\u65e0\u5173\u7684\u57fa\u56e0\uff0c\u84dd\u8272\u70b9\u4ee3\u8868\u4e0eRA\u4ee3\u8c22\u76f8\u5173\u7684\u57fa\u56e0\uff0c\u7eff\u8272\u70b9\u4ee3\u8868RAR\u76ee\u6807\u57fa\u56e0\uff0c\u7c89\u8272\u70b9\u4ee3\u8868\u4e0eRAR\u4fe1\u53f7\u76f8\u5173\u7684\u57fa\u56e0\u3002  <\/p>\n

(G) \u6761\u5f62\u56fe\u5c55\u793a\u4e86PC3shTIMP1\u7ec6\u80de\u5728adapalene\u5904\u7406\u4e0e\u672a\u5904\u7406\u60c5\u51b5\u4e0b\uff0c\u4eceRNA-seq\u5f97\u5230\u7684RAR\u4e0b\u6e38\u76ee\u6807\u3001RA\u76f8\u5173\u4ee3\u8c22\u548cRAR\u4fe1\u53f7\u7684\u6807\u51c6\u5316\u8868\u8fbe\u5206\u6570\uff08NES\uff09\u3002  <\/p>\n

(H) \u56fe\u8868\u5c55\u793a\u4e86\u60a3\u8005\u548c\u7ecfadapalene\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80deRNA-seq\u6570\u636e\u4e2dRA\u76f8\u5173\u57fa\u56e0\u7684\u76ae\u5c14\u68ee\u76f8\u5173\u6027\u3002\u7070\u8272\u70b9\u4ee3\u8868\u4e0eRAR\u901a\u8def\u65e0\u5173\u7684\u57fa\u56e0\uff0c\u9ed1\u8272\u70b9\u4ee3\u8868adapalene\u5904\u7406\u540e\u4e0b\u8c03\u7684\u57fa\u56e0\uff0c\u7ea2\u8272\u70b9\u4ee3\u8868\u4e0a\u8c03\u7684\u57fa\u56e0\u3002  <\/p>\n

(I) (J)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u3002  <\/p>\n

(J) \u5728\u975e\u8f6c\u5316\u524d\u5217\u817a\u7ec6\u80de\u7cfbRWPE-1\u548c\u764c\u7ec6\u80de\u7cfb22RV1\u4e2d\uff0c\u7ecf2<\/sup>H-ROH\u5904\u7406\u540eROH\u8f6c\u5316\u4ea7\u7269\uff08atRA\u548c13-cisRA\uff09\u7684\u500d\u6570\u53d8\u5316\u3002<\/p>\n

\u6570\u636e(J)\u5c55\u793a\u4e3a\u8868\u793a\u4e00\u6b21\u5b9e\u9a8c\u7684\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u3002\u7edf\u8ba1\u6d4b\u8bd5(J)\u4f7f\u7528\u7684\u662f\u5355\u5411ANOVA\u540e\u63a5Tukey\u2019s\u6d4b\u8bd5 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u53e6\u89c1\u56feS2\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 3. RARs\u6fc0\u6d3b\u901a\u8fc7\u91cd\u65b0\u7f16\u7a0bSASP\u589e\u5f3a\u5316\u7597\u6548\u679c<\/strong><\/p>\n

(A) \u70ed\u56fe\u5c55\u793a\u4e86\u5bf9PC3shTIMP1\u7ec6\u80de\u8fdb\u884c\u7684\u534f\u540c\u589e\u6b96\u5b9e\u9a8c\u7ed3\u679c\uff0c\u8fd9\u4e9b\u7ec6\u80de\u63a5\u53d7\u4e86docetaxel\u548cadapalene\u6216docetaxel\u548cbexarotene\u7ec4\u5408\u6cbb\u7597\uff08\u6cbb\u759748\u5c0f\u65f6\uff09\u3002  <\/p>\n

(B) \u5bf9\u7528docetaxel\u3001adapalene\u53ca\u5176\u7ec4\u5408\uff08\u5de6\u4fa7\uff09\u548cdocetaxel\u3001bexarotene\u53ca\u5176\u7ec4\u5408\uff08\u53f3\u4fa7\uff09\u6cbb\u7597\u4e24\u5929\u540e\u7684PC3shTIMP1\u7ec6\u80de\u8fdb\u884c\u7684SA-\u03b2 Gal\u5b9e\u9a8c\u7684\u5b9a\u91cf\u3002  <\/p>\n

(C) \u5bf9\u7528docetaxel\u3001adapalene\u548c\u7ec4\u5408\u836f\u7269\u6cbb\u7597\u7684PC3shTIMP1\u7ec6\u80de\u8fdb\u884c\u8870\u8001\u6807\u5fd7\u7269\u7684RT-qPCR\u5206\u6790\u3002  <\/p>\n

(D) \u5728\u8fde\u7eed12\u5929\u7684\u6cbb\u7597\u4e2d\uff0cPC3shTIMP1\u7ec6\u80de\u589e\u6b96\u7684\u500d\u6570\u53d8\u5316\uff0c\u4ee5\u7b2c7\u5929\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\uff08\u6bcf\u4e09\u5929\u66f4\u6362\u4e00\u6b21\u57f9\u517b\u57fa\uff09\u3002  <\/p>\n

(E) \u5bf9PC3shTIMP1\u7ec6\u80de\u7684RNA-seq\u8fdb\u884c\u7684\u901a\u8def\u5206\u6790\uff0c\u5c55\u793a\u4e86\u7ec4\u5408\u836f\u7269\u4e0edocetaxel\u53cadocetaxel\u4e0e\u5bf9\u7167\u7ec4\u6bd4\u8f83\u65f6\u4e0a\u8c03\u548c\u4e0b\u8c03\u7684\u901a\u8def\u3002\u62a5\u9053\u7684\u901a\u8defFDR <0.05\u3002  <\/p>\n

(F) \u5bf9\u7528\u6307\u5b9a\u836f\u7269\u6cbb\u7597\u7684PC3ShTIMP1\u7ec6\u80de\u8fdb\u884c\u7684\u7ec6\u80de\u56e0\u5b50\u9635\u5217\uff0c\u4ee5\u5bf9\u7167\u7ec4\u7ec6\u80de\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\u3002  <\/p>\n

(G) (H\u548cI)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u3002  <\/p>\n

(H) \u5bf9PC3shTIMP1\u7ec6\u80de\u8fdb\u884c\u7684Boyden\u5ba4\u8fc1\u79fb\u5b9e\u9a8c\u7684\u5b9a\u91cf\uff0c\u8fd9\u4e9b\u7ec6\u80de\u66b4\u9732\u4e8edocetaxel\u3001adapalene\u6216\u7ec4\u5408\u836f\u7269\u7684\u6761\u4ef6\u57f9\u517b\u57fa\u4e2d\uff0c\u4ee5OD590\u7684\u500d\u6570\u53d8\u5316\u8868\u793a\u3002  <\/p>\n

(I) \u7528\u7ed3\u6676\u7d2b\u67d3\u8272\u7684Boyden\u5ba4\u7684\u4ee3\u8868\u6027\u56fe\u50cf\u3002  <\/p>\n

(J) (K\u548cL)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u3002  <\/p>\n

(K) \u7528docetaxel\u3001adapalene\u6216\u7ec4\u5408\u836f\u7269\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u7684\u6761\u4ef6\u57f9\u517b\u57fa\u5904\u7406\u7684PC3shTIMP1\u4eb2\u672c\u7ec6\u80de\u7684\u521b\u4f24\u5bc6\u5ea6\u767e\u5206\u6bd4\u3002  <\/p>\n

(L) K\u7684\u4ee3\u8868\u6027\u56fe\u7247\uff08\u523b\u5ea6\u5c3a\uff1a400\u6beb\u7c73\uff09\u3002  <\/p>\n

(M) (N\u548cO)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u3002  <\/p>\n

(N) \u5728\u4e0e\u8870\u8001\u7684\u672a\u6807\u8bb0RapidCaP\u7ec6\u80de\u5171\u57f9\u517b\u7684\u60c5\u51b5\u4e0b\uff0cTdTomato+ <\/sup>RapidCaP\u7ec6\u80de\u7684\u589e\u6b96\uff0c\u4ee5\u603b\u79ef\u5206\u5f3a\u5ea6\uff08RCU x mm\u00b2\/\u56fe\u50cf\uff09\u7684\u500d\u6570\u53d8\u5316\u91cf\u5316\u3002  <\/p>\n

(O) N\u4e2d\u589e\u6b96\u5b9e\u9a8c\u7684\u4ee3\u8868\u6027\u56fe\u50cf\uff08\u523b\u5ea6\u5c3a\uff1a400\u6beb\u7c73\uff0c\u63d2\u56fe200\u6beb\u7c73\uff09\u3002<\/p>\n

\u6570\u636e(B)\u3001(C)\u548c(H)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u663e\u793a\u3002\u6570\u636e(H)\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u5355\u5411ANOVA\u540e\u63a5Tukey\u2019s\u6d4b\u8bd5 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e(D)\u3001(K)\u548c(N)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u663e\u793a\u3002\u6570\u636e(B\u2013D)\u3001(K)\u548c(N)\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u53cc\u5411ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e(F)\u4ee3\u8868\u4e00\u6b21\u5b9e\u9a8c\u3002\u56fe\u7248(A\u2013D)\u3001(H)\u3001(K)\u548c(N)\u7684\u6570\u636e\u4ee3\u8868\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002\u53e6\u89c1\u56feS3\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 4. \u963f\u8fbe\u5e15\u6797\u901a\u8fc7AP-1\u91cd\u65b0\u7f16\u7a0b\u7531\u591a\u897f\u4ed6\u8d5b\u5f15\u53d1\u7684\u6709\u5bb3SASP<\/strong><\/p>\n

(A) \u70ed\u56fe\u5c55\u793a\u4e86\u7528\u591a\u897f\u4ed6\u8d5b\u3001\u963f\u8fbe\u5e15\u6797\u548c\u7ec4\u5408\u836f\u7269\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u4e2d\u5dee\u5f02\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\u3002\u81f3\u5c11\u5728\u4e00\u6b21\u4e0e\u5bf9\u7167\u836f\u7269\u6bd4\u8f83\u4e2d\u62a5\u544a\u7684\u57fa\u56e0FDR <0.05\u3002  <\/p>\n

(B) \u70ed\u56fe\u7684\u7126\u70b9\u5c55\u793a\u4e86\u5728PC3shTIMP1\u7ec6\u80de\u4e2d\u5dee\u5f02\u8868\u8fbe\u7684\u8f6c\u5f55\u56e0\u5b50\uff0c\u8fd9\u4e9b\u56e0\u5b50\u5728\u7528\u591a\u897f\u4ed6\u8d5b\u4e0e\u5bf9\u7167\u836f\u7269\u5904\u7406\u7684\u7ec6\u80de\u4ee5\u53ca\u7ec4\u5408\u836f\u7269\u4e0e\u591a\u897f\u4ed6\u8d5b\u5904\u7406\u7684\u7ec6\u80de\u4e2d\u6709\u76f8\u53cd\u7684\u8868\u8fbe\u6a21\u5f0f\u3002  <\/p>\n

(C) \uff08D\u548cE\uff09\u7684\u793a\u610f\u56fe\u3002  <\/p>\n

(D) \u4ee3\u8868\u6027\u56fe\u7247\u5c55\u793a\u4e86\u4ee5mCherry\u8367\u5149\u8868\u793a\u7684AP-1\u4ecb\u5bfc\u7684\u8f6c\u5f55\u6fc0\u6d3b\u3002  <\/p>\n

(E) \u5728\u6709\u6216\u65e0AP-1\u6291\u5236\u5242\uff08AP-1i\uff09T-5224\u7684\u60c5\u51b5\u4e0b\uff0c\u7528\u591a\u897f\u4ed6\u8d5b\u3001\u963f\u8fbe\u5e15\u6797\u6216\u7ec4\u5408\u836f\u7269\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u4e2dmCherry\u9633\u6027\u7ec6\u80de\u7684\u5b9a\u91cf\u3002  <\/p>\n

(F) \u5728\u6709\u6216\u65e0AP-1\u6291\u5236\u5242\u7684\u60c5\u51b5\u4e0b\uff0c\u7528\u591a\u897f\u4ed6\u8d5b\u3001\u963f\u8fbe\u5e15\u6797\u6216\u7ec4\u5408\u836f\u7269\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u7684SA-\u03b2 Gal\u67d3\u8272\u5b9a\u91cf\u3002  <\/p>\n

(G) \u5b9e\u9a8c\u8bbe\u8ba1\u7684\u8868\u793a\uff08\u4e0a\u56fe\uff09\u548c\u5728\u6709\u6216\u65e0AP-1\u6291\u5236\u5242\u7684\u60c5\u51b5\u4e0b\uff0c\u7528\u524d\u5217\u817a\u80bf\u7624\u7ec6\u80de\u7684\u6761\u4ef6\u57f9\u517b\u57fa\uff08\u7ecf\u591a\u897f\u4ed6\u8d5b\u6216\u7ec4\u5408\u836f\u7269\u5904\u7406\uff09\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u7684\u589e\u6b96\u500d\u6570\u53d8\u5316\uff0c\u4ee5t0\u4e3a\u57fa\u51c6\uff08\u4e0b\u56fe\uff09\u3002  <\/p>\n

(H) \u5b9e\u9a8c\u8bbe\u8ba1\uff08\u4e0a\u56fe\uff09\u548c\u7528\u7ecf\u591a\u897f\u4ed6\u8d5b\u548c\u7ec4\u5408\u836f\u7269\u5904\u7406\u7684\u7ec6\u80de\u7684\u6761\u4ef6\u57f9\u517b\u57fa\u5904\u7406\u7684PC3shTIMP1\u7ec6\u80de\u7684\u521b\u4f24\u5e76\u5408\u767e\u5206\u6bd4\uff08\u4e0b\u56fe\uff09\u3002  <\/p>\n

(I) (H)\u4e2d\u521b\u4f24\u6108\u5408\u5b9e\u9a8c\u7684\u4ee3\u8868\u6027\u56fe\u7247\uff08\u523b\u5ea6\u5c3a\uff1a400\u6beb\u7c73\uff09\u3002  <\/p>\n

(J) \u6240\u793a\u80bf\u7624\u4fc3\u8fdb\u5206\u6ccc\u56e0\u5b50mRNA\u6c34\u5e73\u7684\u500d\u6570\u53d8\u5316\u3002  <\/p>\n

(K) \u4e0d\u540c\u6297\u80bf\u7624\u514d\u75ab\u53cd\u5e94\u6fc0\u6d3b\u56e0\u5b50mRNA\u6c34\u5e73\u7684\u500d\u6570\u53d8\u5316\u3002<\/p>\n

\u6570\u636e(G)\u548c(H)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u663e\u793a\u3002\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u53cc\u5411ANOVA\uff0c****p < 0.0001\u3002\u6570\u636e\u4ee3\u8868\u4e09\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002\u6570\u636e(E)\u3001(F)\u3001(J)\u548c(K)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u663e\u793a\u3002\u6570\u636e(E)\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u5355\u5411ANOVA\u540e\u63a5Tukey\u2019s\u6d4b\u8bd5 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e(F\u2013H)\u3001(J)\u548c(K)\uff1a\u53cc\u5411ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e\u4ee3\u8868\u4e24\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002\u53e6\u89c1\u56feS4\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\n

\u56fe 5. \u963f\u8fbe\u5e15\u6797\u8054\u5408\u591a\u897f\u4ed6\u8d5b\u589e\u5f3a\u7ec6\u80de\u8870\u8001\u5e76\u6fc0\u6d3b\u6297\u80bf\u7624\u514d\u75ab\u53cd\u5e94<\/strong><\/p>\n

(A) \u5b9e\u9a8c\u8bbe\u8ba1\u3002  <\/p>\n

(B) \u7ed9\u4e88\u6307\u5b9a\u836f\u7269\u6cbb\u7597\u7684\u5c0f\u9f20\u7684\u80bf\u7624\u4f53\u79ef\uff08\u5355\u4f4d\uff1amm\u00b3\uff0c\u6bcf\u7ec4n = 5\uff09\uff08\u5de6\u56fe\uff09\uff0c\u4ee5\u53ca\u57fa\u4e8e\u80bf\u7624\u4f53\u79ef\u8ba1\u7b97\u7684\u66f2\u7ebf\u4e0b\u9762\u79ef\uff08\u53f3\u56fe\uff09\u3002  <\/p>\n

(C) \u80bf\u7624\u5207\u7247\u4e2dNK\u7ec6\u80de\uff08\u4f7f\u7528\u6297NK1.1\u6297\u4f53\uff0c\u7ea2\u8272\uff09\u548c\u88c2\u89e3\u578bcaspase 3\uff08CC3\uff0c\u7eff\u8272\uff09\u7684\u514d\u75ab\u8367\u5149\u67d3\u8272\uff08\u523b\u5ea6\u5c3a\uff1a21 mm\uff09\u3002  <\/p>\n

(D) \u6765\u81ea(C)\u4e2d\u80bf\u7624\u5207\u7247\u6bcf\u4e2a\u89c6\u91ce\u4e2dCC3\u9633\u6027\u7ec6\u80de\u7684\u5b9a\u91cf\u3002  <\/p>\n

(E) \u7528\u6307\u5b9a\u836f\u7269\u6cbb\u7597\u7684\u80bf\u7624\u4e2dNK1.1\u548cCD45+<\/sup>\u7ec6\u80de\u7684FACS\u56fe\uff0c\u7b5b\u9009\u6d3b\u7ec6\u80de\u3002  <\/p>\n

(F) \u7528\u6307\u5b9a\u836f\u7269\u6cbb\u7597\u7684\u80bf\u7624\u4e2dNK1.1+<\/sup>\u7ec6\u80de\u7684\u767e\u5206\u6bd4\u3002  <\/p>\n

(G) \u80bf\u7624\u5207\u7247\u4e2dNK\u7ec6\u80de\uff08\u4f7f\u7528\u6297NKp46\u6297\u4f53\uff0c\u7eff\u8272\uff09\u7684\u514d\u75ab\u8367\u5149\u67d3\u8272\uff08\u523b\u5ea6\u5c3a\uff1a33.1 mm\uff09\u3002  <\/p>\n

(H) \u6765\u81ea(G)\u7684\u80bf\u7624\u5207\u7247\u6bcf\u4e2a\u89c6\u91ce\u4e2dNK\u7ec6\u80de\u7684\u5b9a\u91cf\u3002  <\/p>\n

(I) \u7528\u6307\u5b9a\u836f\u7269\u6cbb\u7597\u7684\u80bf\u7624\u4e2d\u62db\u52dfNK\u7ec6\u80de\u53ca\u5176\u4ea7\u751f\u7684\u9897\u7c92\u9176B\u548cIFN\u03b3\u7684mRNA\u6c34\u5e73\u7684\u500d\u6570\u53d8\u5316\u3002  <\/p>\n

(J) (K\u548cL)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u793a\u610f\u56fe\u3002  <\/p>\n

(K) \u672a\u7ecf\u6cbb\u7597\u6216\u7528\u8054\u5408\u836f\u7269\u548c\u6297GM1 Asialo\u6cbb\u7597\u7684\u5c0f\u9f20\u80bf\u7624\u4e2d\u6d78\u6da6\u7684NK\u7ec6\u80de\uff08NK1.1+<\/sup>\uff0cCD3–<\/sup>\uff0cNKp46+<\/sup>\uff09\u5728CD45+<\/sup>\u7ec6\u80de\u4e2d\u7684\u767e\u5206\u6bd4\u3002  <\/p>\n

(L) \u5728\u6709\u6216\u65e0NK\u7ec6\u80de\u8017\u7aed\u6297\u4f53\u5b58\u5728\u4e0b\uff0c\u7528\u6307\u5b9a\u836f\u7269\u6cbb\u7597\u7684\u5c0f\u9f20\u7684\u80bf\u7624\u4f53\u79ef\uff08\u5355\u4f4d\uff1amm\u00b3\uff0cVehicle, Iso n = 4, Vehicle, Asialo n = 4, Combo, Iso n = 3, Combo, Asialo n = 4\uff09\u3002<\/p>\n

\u6570\u636e(B)\u548c(L)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u663e\u793a\u3002\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u53cc\u5411ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e(B)\u4ee3\u8868\u4e24\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002\u6570\u636e(L)\u4ee3\u8868\u4e00\u6b21\u5b9e\u9a8c\u3002\u6570\u636e(D)\u3001(F)\u3001(H)\u3001(I)\u548c(K)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u663e\u793a\u3002\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u5355\u5411ANOVA\u540e\u63a5Tukey\u2019s\u6d4b\u8bd5 *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e\u4ee3\u8868\u4e00\u6b21\u5b9e\u9a8c\u3002\u53e6\u89c1\u56feS5\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u56fe 6. RAR\u6fc0\u52a8\u5242\u8054\u5408\u591a\u897f\u4ed6\u8d5b\u589e\u5f3a\u4e86\u6e90\u81ea\u4eba\u4f53\u7684NK\u7ec6\u80de\u7684\u6297\u80bf\u7624\u6d3b\u6027<\/strong><\/p>\n

(A) \u5b9e\u9a8c\u8bbe\u8ba1\u3002  <\/p>\n

(B) \u4ee3\u8868\u6027\u56fe\u50cf\u663e\u793a\u4e0e\u4ebaNK\u7ec6\u80de\u5171\u57f9\u517b\u7684\u3001\u9884\u5148\u7528\u591a\u897f\u4ed6\u8d5b\u548c\u7ec4\u5408\u836f\u7269\u5904\u7406\u8fc7\u7684PC3\u7ec6\u80de\u7684\u7ec6\u80de\u6bd2\u6027\u53d1\u5c04\uff08\u7eff\u8272\uff09\uff08\u523b\u5ea6\u5c3a\uff1a400mm\uff09\u3002  <\/p>\n

(C) PC3\u7ec6\u80de\u5728\u9884\u5904\u7406\u6307\u5b9a\u5316\u5408\u7269\u540e\u4e0e\u4ebaNK\u7ec6\u80de\u5171\u57f9\u517b\uff08\u76ee\u6807\uff1a\u6548\u5e94\u6bd41:5\uff09\u65f6\uff0c\u7ec6\u80de\u6bd2\u6027\u53d1\u5c04\u7684\u5b9a\u91cf\uff0c\u4ee5\u603b\u79ef\u5206\u5f3a\u5ea6\uff08GCU x mm\u00b2\/\u56fe\u50cf\uff09\u7684\u500d\u6570\u53d8\u5316\u8868\u793a\u3002  <\/p>\n

(D) LNCaP\u7ec6\u80de\u5728\u9884\u5904\u7406\u6307\u5b9a\u5316\u5408\u7269\u540e\u4e0e\u4ebaNK\u7ec6\u80de\u5171\u57f9\u517b\uff08\u76ee\u6807\uff1a\u6548\u5e94\u6bd41:5\uff09\u65f6\uff0c\u7ec6\u80de\u6bd2\u6027\u53d1\u5c04\u7684\u5b9a\u91cf\uff0c\u4ee5\u603b\u79ef\u5206\u5f3a\u5ea6\uff08GCU x mm\u00b2\/\u56fe\u50cf\uff09\u7684\u500d\u6570\u53d8\u5316\u8868\u793a\u3002  <\/p>\n

(E) 22RV1\u7ec6\u80de\u5728\u9884\u5904\u7406\u6307\u5b9a\u5316\u5408\u7269\u540e\u4e0e\u4ebaNK\u7ec6\u80de\u5171\u57f9\u517b\uff08\u76ee\u6807\uff1a\u6548\u5e94\u6bd41:5\uff09\u65f6\uff0c\u7ec6\u80de\u6bd2\u6027\u53d1\u5c04\u7684\u5b9a\u91cf\uff0c\u4ee5\u603b\u79ef\u5206\u5f3a\u5ea6\uff08GCU x mm\u00b2\/\u56fe\u50cf\uff09\u7684\u500d\u6570\u53d8\u5316\u8868\u793a\u3002  <\/p>\n

(F) (G)\u7684\u5b9e\u9a8c\u5e03\u5c40\u793a\u610f\u56fe\u3002  <\/p>\n

(G) PC3\u7ec6\u80de\u7ecf\u6307\u5b9a\u5316\u5408\u7269\u5904\u7406\u540e\u7684caspase 3\/7\u7d2f\u79ef\u8367\u5149\u5f3a\u5ea6\uff0c\u4ee5\u65f6\u95f40\uff08t0\uff09\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\uff0c\u5de6\u56fe\u4e3a\u4e0d\u540c\u5316\u5408\u7269\u5904\u7406\uff0c\u53f3\u56fe\u4e3a\u4e0d\u540cshRNAs\u5de5\u7a0b\u5316\u5904\u7406\uff1aEV\uff08\u5706\u5708\uff09\u3001shIL-33\uff08\u65b9\u5757\uff09\u3001ShIL-12\uff08\u4e09\u89d2\uff09\u3002  <\/p>\n

(H) PC3\u7ec6\u80de\u7684RT-qPCR\u5206\u6790\uff0c\u663e\u793a\u764c\u7ec6\u80de\u4e0aNK\u914d\u4f53\u7684\u8868\u8fbe\u3002  <\/p>\n

(I) FACS\u5206\u6790\u663e\u793a\u8868\u8fbeULBP2\u7684PC3\u7ec6\u80de\u7684\u9633\u6027\u767e\u5206\u6bd4\u3002  <\/p>\n

(J) FACS\u5206\u6790\u663e\u793a\u8868\u8fbeMICA\u7684PC3\u7ec6\u80de\u7684\u9633\u6027\u767e\u5206\u6bd4\u3002  <\/p>\n

(K) (L)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u793a\u610f\u56fe\u3002  <\/p>\n

(L) \u5728\u6709\u6216\u65e0\u4e2d\u548cNKG2D\u6297\u4f53\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u7528\u6307\u5b9a\u5316\u5408\u7269\u5904\u7406\u7684PC3\u7ec6\u80de\u7684Cytotox\u7eff\u8272\u53d1\u5c04\u79ef\u5206\u5f3a\u5ea6\uff0c\u4ee5\u65f6\u95f40\uff08t0\uff09\u4e3a\u57fa\u51c6\u8fdb\u884c\u6807\u51c6\u5316\u3002  <\/p>\n

(M) (N\u548cO)\u7684\u5b9e\u9a8c\u8bbe\u8ba1\u8868\u793a\u3002  <\/p>\n

(N) \u7ecf\u4e0d\u540c\u5904\u7406\u7684\u5c0f\u9f20\u7684\u80bf\u7624\u4f53\u79ef\uff08mm\u00b3\uff09\uff0c\u5206\u7ec4\u60c5\u51b5\uff1a\u5bf9\u7167\uff08n = 15\uff09\uff0c\u5bf9\u7167+NK\uff08n = 16\uff09\uff0c\u591a\u897f\u4ed6\u8d5b\uff08n = 7\uff09\uff0c\u591a\u897f\u4ed6\u8d5b+NK\uff08n = 7\uff09\uff0c\u7ec4\u5408\uff08n = 15\uff09\uff0c\u7ec4\u5408+NK\uff08n = 16\uff09\u3002\u7531\u4e8e\u80bf\u7624\u6e83\u75a1\uff0c\u5bf9\u7167\u7ec4\u3001\u5bf9\u7167NK\u7ec4\u3001\u591a\u897f\u4ed6\u8d5b\u7ec4\u548c\u591a\u897f\u4ed6\u8d5bNK\u7ec4\u7684\u5c0f\u9f20\u5728\u7b2c44\u5929\u8f83\u65e9\u65f6\u95f4\u70b9\u88ab\u5b89\u4e50\u6b7b\u3002  <\/p>\n

(O) FACS\u5206\u6790\u663e\u793a\u95e8\u63a7\u5728hCD45+\u3001mCD45-\u4e2d\u7684hCD56+\u7ec6\u80de\u7684\u767e\u5206\u6bd4\u3002<\/p>\n

\u6570\u636e(G)\u3001(I)\u3001(J)\u548c(O)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u5dee\u663e\u793a\u3002\u6570\u636e(C\u2013E)\u3001(L)\u548c(N)\u4ee5\u5747\u503c \u00b1 \u6807\u51c6\u8bef\u5dee\u663e\u793a\u3002\u6570\u636e(C\u2013E)\u3001(G)\u548c(N)\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u53cc\u5411ANOVA *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001\u3002\u6570\u636e(I)\u548c(J)\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u5355\u5411ANOVA\u540e\u63a5Tukey\u2019s\u6d4b\u8bd5 ***p < 0.001\u3002\u6570\u636e(C\u2013E)\u3001(G\u2013J)\u3001(L)\u548c(N)\u4ee3\u8868\u4e24\u6b21\u72ec\u7acb\u5b9e\u9a8c\u3002\u6570\u636e(O)\u4ee3\u8868\u4e00\u6b21\u5b9e\u9a8c\u3002\u6570\u636e(L)\u4f7f\u7528\u7684\u7edf\u8ba1\u6d4b\u8bd5\uff1a\u53cc\u5411ANOVA\u3002\u5e26\u6709\u4e0d\u540c\u7b26\u53f7\u7684\u7ebf\u8868\u793a\u5f7c\u6b64\u95f4\u7edf\u8ba1\u5b66\u4e0a\u5b58\u5728\u663e\u8457\u5dee\u5f02\u3002\u53e6\u89c1\u56feS6\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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OncoLab\u516c\u4f17\u53f7\u5df2\u589e\u52a0\u671f\u520a\u67e5\u8be2\u529f\u80fd<\/span><\/strong>\uff0cSCI\u671f\u520a<\/strong><\/span>\u53ef\u67e5\u8be2\u671f\u520a\u540d\u79f0\u7f29\u5199\u3001ISSN\u520a\u53f7\u3001\u8fd1\u4e94\u5e74\u5f71\u54cd\u56e0\u5b50<\/strong>\u3001\u6700\u65b0WOS\u7684JCR\u5206\u533a<\/strong>\u3001\u6700\u65b0\u4e2d\u79d1\u9662\u5206\u533a<\/strong>\u4ee5\u53ca\u4e2d\u79d1\u9662\u671f\u520a\u9884\u8b66\u4fe1\u606f<\/strong>\uff1b<\/p>\n

\u4e2d\u6587\u671f\u520a<\/span><\/strong>\u53ef\u68c0\u7d22CSCD\u6838\u5fc3<\/strong>\u3001\u5317\u5927\u4e2d\u6587\u6838\u5fc3<\/strong>\u3001\u79d1\u6280\u6838\u5fc3<\/strong>\u6536\u5f55\u60c5\u51b5\uff1b<\/p>\n

\u5728\u516c\u4f17\u53f7\u5bf9\u8bdd\u6846\u53d1\u9001\u6742\u5fd7\u540d\u79f0<\/span><\/strong>\u5373\u53ef\u5feb\u901f\u68c0\u7d22\uff0c\u652f\u6301\u6a21\u7cca\u68c0\u7d22\uff0c\u6b22\u8fce\u4f7f\u7528\uff01<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\u2014 THE  END \u2014<\/span><\/section>\n
\u6765\u6e90 | Cancer Cell<\/span><\/section>\n
\u7b5b\u9009\u6574\u7406 | \u738b\u5764<\/span><\/span><\/span><\/section>\n
\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u53ef\u8f6c\u5230\u6587\u7ae0\u9875\u9762
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\u516c\u4f17\u53f7\u53d1\u90012024022705\u53ef\u83b7\u53d6\u6587\u732e\u5168\u6587<\/span><\/span><\/span><\/section>\n


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\u2589  \u5f80\u671f\u63a8\u9001\u5408\u8f91<\/strong><\/strong><\/section>\n
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\u80bf\u7624\u514d\u75ab\u5b66\u6700\u65b0\u6587\u732e<\/span><\/a>
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\u514d\u75ab\u5b66\u62fe\u9057\u7cfb\u5217<\/a><\/span><\/p>\n

\u80bf\u7624\u514d\u75ab\u5b66\u7cfb\u5217<\/span><\/a><\/p>\n

ACIR\u6587\u732e\u901f\u9012<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u4f5c\u56fe<\/span><\/a><\/p>\n

SCI\u8bba\u6587\u5199\u4f5c\u53ca\u6295\u7a3f<\/span><\/a><\/p>\n

\u751f\u4fe1\u5165\u95e8\u5b66\u4e60\u7b14\u8bb0<\/span><\/a><\/p>\n

R\u8bed\u8a00\u57fa\u7840\u4e0e\u5355\u7ec6\u80de\u5206\u6790\u5b9e\u6218\u89c6\u9891\u8bfe\u7a0b<\/span><\/a><\/p>\n

\u751f\u7269\u5b66\u5b9e\u9a8c\u6280\u672f\u53ca\u5e38\u7528\u8f6f\u4ef6<\/span><\/a><\/p>\n

RCIM\u4eea\u5668\u7b80\u4ecb\u53ca\u64cd\u4f5c\u89c6\u9891<\/a><\/span><\/p>\n

\u5e38\u7528\u7535\u8111\u8f6f\u4ef6\u4f7f\u7528\u6559\u7a0b<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u52a0\u5165\u8bfb\u8005\u4ea4\u6d41\u7fa4~<\/span><\/p>\n

\uff08\u6dfb\u52a0\u8bf7\u5907\u6ce8\u5355\u4f4d\u59d3\u540d\uff09<\/span><\/p>\n


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\u70b9\u4eae\u8d5e\u4e0e\u5728\u770b <\/span><\/p>\n

\u8ba9\u66f4\u591a\u4eba\u770b\u5230<\/span><\/p>\n

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\u672c\u7bc7\u6587\u7ae0\u6765\u6e90\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7: OncoLab <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u524d\u671f\u7cbe\u5f69\u5185\u5bb9\uff1a Nat Commun\uff1a\u8870\u8001\u901a\u8fc7\u8bf1\u5bfc\u514d\u75ab\u6291\u5236\u6027\u80bf\u7624\u5fae\u73af\u5883\u5bfc\u81f4\u514d\u75ab\u6cbb\u7597\u6297\u6027 Cancer Cell…<\/p>\n","protected":false},"author":1,"featured_media":3435,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[63],"tags":[],"_links":{"self":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/3443"}],"collection":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/comments?post=3443"}],"version-history":[{"count":0,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/posts\/3443\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/media\/3435"}],"wp:attachment":[{"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/media?parent=3443"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/categories?post=3443"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/oncolab.cn\/index.php\/wp-json\/wp\/v2\/tags?post=3443"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}